Sevoflurane depresses neurons in the medial parabrachial nucleus by potentiating postsynaptic GABA
Anesthetics, Inhalation
/ pharmacology
Animals
Electrophysiological Phenomena
GABA Antagonists
/ pharmacology
Male
Membrane Potentials
/ drug effects
Mice
Mice, Inbred C57BL
Nerve Fibers
/ drug effects
Neurons
/ drug effects
Parabrachial Nucleus
/ drug effects
Patch-Clamp Techniques
Picrotoxin
/ pharmacology
Potassium Channels
/ drug effects
Receptors, GABA-A
/ drug effects
Sevoflurane
/ antagonists & inhibitors
GABA(A) receptors
Medial parabrachial nucleus
Potassium channels
Sevoflurane
Journal
Neuropharmacology
ISSN: 1873-7064
Titre abrégé: Neuropharmacology
Pays: England
ID NLM: 0236217
Informations de publication
Date de publication:
15 12 2020
15 12 2020
Historique:
received:
12
01
2020
revised:
23
06
2020
accepted:
20
07
2020
pubmed:
16
9
2020
medline:
24
9
2021
entrez:
15
9
2020
Statut:
ppublish
Résumé
Despite persistent clinical use for over 170 years, the neuronal mechanisms by which general anesthetics produce hypnosis remain unclear. Previous studies suggest that anesthetics exert hypnotic effects by acting on endogenous arousal circuits. Recently, it has been shown that the medial parabrachial nucleus (MPB) is a novel wake-promoting component in the dorsolateral pons. However, it is not known whether and how the MPB contributes to anesthetic-induced hypnosis. Here, we investigated the action of sevoflurane, a widely used volatile anesthetic agent that best represents the drug class of halogenated ethers, on MPB neurons in mice. Using in vivo fiber photometry, we found that the population activities of MPB neurons were inhibited during sevoflurane-induced loss of consciousness. Using in vitro whole-cell patch-clamp recordings, we revealed that sevoflurane suppressed the firing rate of MPB neurons in concentration-dependent and reversible manners. At a concentration equal to MAC of hypnosis, sevoflurane potentiated synaptic GABA
Identifiants
pubmed: 32931816
pii: S0028-3908(20)30317-8
doi: 10.1016/j.neuropharm.2020.108249
pii:
doi:
Substances chimiques
Anesthetics, Inhalation
0
GABA Antagonists
0
Potassium Channels
0
Receptors, GABA-A
0
Picrotoxin
124-87-8
Sevoflurane
38LVP0K73A
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
108249Informations de copyright
Copyright © 2020. Published by Elsevier Ltd.