Contribution of Staphylococcal Enterotoxin B to Staphylococcus aureus Systemic Infection.

Staphylococcus aureus CA-MRSA ST59 cytokine storm sepsis staphylococcal enterotoxin B superantigen

Journal

The Journal of infectious diseases
ISSN: 1537-6613
Titre abrégé: J Infect Dis
Pays: United States
ID NLM: 0413675

Informations de publication

Date de publication:
28 05 2021
Historique:
received: 29 04 2020
accepted: 14 09 2020
pubmed: 17 9 2020
medline: 12 2 2022
entrez: 16 9 2020
Statut: ppublish

Résumé

Staphylococcal enterotoxin B (SEB), which is produced by the major human pathogen, Staphylococcus aureus, represents a powerful superantigenic toxin and is considered a bioweapon. However, the contribution of SEB to S. aureus pathogenesis has never been directly demonstrated with genetically defined mutants in clinically relevant strains. Many isolates of the predominant Asian community-associated methicillin-resistant S. aureus lineage sequence type (ST) 59 harbor seb, implying a significant role of SEB in the observed hypervirulence of this lineage. We created an isogenic seb mutant in a representative ST59 isolate and assessed its virulence potential in mouse infection models. We detected a significant contribution of seb to systemic ST59 infection that was associated with a cytokine storm. Our results directly demonstrate that seb contributes to S. aureus pathogenesis, suggesting the value of including SEB as a target in multipronged antistaphylococcal drug development strategies. Furthermore, they indicate that seb contributes to fatal exacerbation of community-associated methicillin-resistant S. aureus infection.

Identifiants

pubmed: 32937658
pii: 5906645
doi: 10.1093/infdis/jiaa584
pmc: PMC8161638
doi:

Substances chimiques

Enterotoxins 0
enterotoxin B, staphylococcal 39424-53-8

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, N.I.H., Intramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1766-1775

Subventions

Organisme : NIAID NIH HHS
ID : R21 AI142243
Pays : United States
Organisme : Intramural NIH HHS
ID : ZIA AI000904
Pays : United States

Informations de copyright

Published by Oxford University Press for the Infectious Diseases Society of America 2020.

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Auteurs

Justin S Bae (JS)

Laboratory of Bacteriology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.

Fei Da (F)

Laboratory of Bacteriology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.

Ryan Liu (R)

Laboratory of Bacteriology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.

Lei He (L)

Department of Laboratory Medicine, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.

Huiying Lv (H)

Department of Laboratory Medicine, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.

Emilie L Fisher (EL)

Laboratory of Bacteriology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.

Govindarajan Rajagopalan (G)

Mayo Clinic College of Medicine, Rochester, Minnesota, USA.

Min Li (M)

Department of Laboratory Medicine, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.

Gordon Y C Cheung (GYC)

Laboratory of Bacteriology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.

Michael Otto (M)

Laboratory of Bacteriology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.

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Classifications MeSH