Bilayer compositional asymmetry influences the nanoscopic to macroscopic phase domain size transition.
Asymmetry
Coarse grained
Lipid domain
Phase separation
Raft
United atom
Journal
Chemistry and physics of lipids
ISSN: 1873-2941
Titre abrégé: Chem Phys Lipids
Pays: Ireland
ID NLM: 0067206
Informations de publication
Date de publication:
10 2020
10 2020
Historique:
received:
28
04
2020
revised:
18
08
2020
accepted:
04
09
2020
pubmed:
18
9
2020
medline:
30
6
2021
entrez:
17
9
2020
Statut:
ppublish
Résumé
The eukaryotic plasma membrane (PM) exhibits lipid mixing heterogeneities known as lipid rafts. These lipid rafts, the result of liquid-liquid phase separation, can be modeled by coexisting liquid ordered (Lo) and liquid disordered (Ld) domains. Four-lipid component systems with a high-melting lipid, a nanodomain-inducing low-melting lipid, a macrodomain-inducing low-melting lipid, and cholesterol (chol) can give rise to domains of different sizes. These four-component systems have been characterized in experiments, yet there are few studies that model the asymmetric distribution of lipids actually found in the PM. We used molecular dynamics (MD) simulations to analyze the transition from nanoscopic to macroscopic domains in symmetric and in asymmetric model membranes. Using coarse-grained MD simulations, we found that asymmetry promotes macroscopic domain growth in a case where symmetric systems exhibit nanoscopic domains. Also, macroscopic domain formation in symmetric systems is highly dependent on registration of like phases in the cytoplasmic and exoplasmic leaflets. Using united-atom MD simulations, we found that symmetric Lo domains are only slightly more ordered than asymmetric Lo domains. We also found that large Lo domains in our asymmetric systems induce a slight chain ordering in the apposed cytoplasmic regions. The chol fractions of phase-separated Lo and Ld domains of the exoplasmic leaflet were unchanged whether the system was symmetric or asymmetric.
Identifiants
pubmed: 32941827
pii: S0009-3084(20)30103-1
doi: 10.1016/j.chemphyslip.2020.104972
pmc: PMC9070412
mid: NIHMS1628962
pii:
doi:
Substances chimiques
Lipid Bilayers
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
104972Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM105684
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM008267
Pays : United States
Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.
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