A Novel Method for Measuring Serum Unbound Bilirubin Levels Using Glucose Oxidase-Peroxidase and Bilirubin-Inducible Fluorescent Protein (UnaG): No Influence of Direct Bilirubin.
Artifacts
Bilirubin
/ blood
Equipment Design
Fetal Blood
/ chemistry
Gestational Age
Glucose Oxidase
Humans
Hyperbilirubinemia, Neonatal
/ blood
Liver Function Tests
/ instrumentation
Oxidation-Reduction
Peroxidase
Reproducibility of Results
Retrospective Studies
Serum
/ chemistry
Spectrometry, Fluorescence
/ instrumentation
Spectrophotometry
/ instrumentation
albumin
analyzer
bilirubin encephalopathy
glucose oxidase-peroxidase method
indirect bilirubin
neonatal intensive care unit
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
16 Sep 2020
16 Sep 2020
Historique:
received:
26
08
2020
revised:
12
09
2020
accepted:
14
09
2020
entrez:
19
9
2020
pubmed:
20
9
2020
medline:
27
2
2021
Statut:
epublish
Résumé
The glucose oxidase-peroxidase (GOD-POD) method used to measure serum unbound bilirubin (UB) suffers from direct bilirubin (DB) interference. Using a bilirubin-inducible fluorescent protein from eel muscle (UnaG), a novel GOD-POD-UnaG method for measuring UB was developed. Newborn sera with an indirect bilirubin/albumin (iDB/A) molar ratio of <0.5 were classified into four groups of DB/total serum bilirubin (TB) ratios (<5%, 5-10%, 10-20%, and ≥20%), and the correlation between the UB levels and iDB/A ratio was examined. Linear regression analysis was performed to compare UB values from both methods with the iDB/A ratio from 38 sera samples with DB/TB ratio <5% and 11 samples with DB/TB ratio ≥5%. The correlation coefficient (
Identifiants
pubmed: 32947818
pii: ijms21186778
doi: 10.3390/ijms21186778
pmc: PMC7555467
pii:
doi:
Substances chimiques
Glucose Oxidase
EC 1.1.3.4
Peroxidase
EC 1.11.1.7
Bilirubin
RFM9X3LJ49
Types de publication
Comparative Study
Journal Article
Validation Study
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Japan Society for the Promotion of Science
ID : 15K19652 and 20K08239
Organisme : Japan Agency for Medical Research and Development
ID : JP19ek0109265
Organisme : Nihon University Research Grant for Social Implementation
ID : Sha19-009
Organisme : Morinaga Hoshi-kai Foundation
ID : -
Organisme : Kurozumi Medical Foundation
ID : -
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