Cannabidiol Modifies the Formation of NETs in Neutrophils of Psoriatic Patients.
Adult
Cannabidiol
/ pharmacology
Case-Control Studies
Cell-Free Nucleic Acids
/ analysis
Culture Media, Conditioned
/ chemistry
Drug Synergism
Enzyme Induction
/ drug effects
Extracellular Traps
/ drug effects
Female
Humans
Lipopolysaccharides
/ pharmacology
Male
NADPH Oxidases
/ biosynthesis
Neutrophils
/ drug effects
Oxidation-Reduction
Peroxidase
/ analysis
Psoriasis
/ blood
Reactive Oxygen Species
/ metabolism
NETs
cannabidiol treatment
immunity
neutrophil traps
psoriasis
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
16 Sep 2020
16 Sep 2020
Historique:
received:
15
08
2020
revised:
11
09
2020
accepted:
15
09
2020
entrez:
19
9
2020
pubmed:
20
9
2020
medline:
27
2
2021
Statut:
epublish
Résumé
Psoriasis is associated with increased production of reactive oxygen species which leads to oxidative stress. As antioxidants can provide protection, the aim of this study was to evaluate the effects of cannabidiol (CBD) on neutrophil extracellular trap (NET) formation in psoriatic and healthy neutrophils. Important markers of NETosis were measured in healthy and psoriatic neutrophils after incubation with CBD, lipopolysaccharide (LPS), and LPS + CBD). The percentage of neutrophils undergoing NETosis and the level of NETosis markers (cfDNA, MPO, elastase) were higher in the neutrophils and blood plasma of psoriatic patients, compared to controls. After LPS treatment, all of the markers of NETosis, except elastase, and p47 and citrullinated histones, were increased in samples from healthy subjects and psoriasis patients. CBD reduced the concentrations of NETosis markers. This led to a reduction in NETosis, which was more pronounced in psoriatic neutrophils and neutrophils treated with LPS in both psoriatic and healthy participants. These results suggest that psoriatic patients neutrophils are at a higher risk of NETosis both in vitro and in vivo. CBD reduces NETosis, mainly in psoriatic neutrophils, possibly due to its antioxidant properties. The anti-NET properties of CBD suggest the positive effect of CBD in the treatment of autoimmune diseases.
Identifiants
pubmed: 32947961
pii: ijms21186795
doi: 10.3390/ijms21186795
pmc: PMC7554718
pii:
doi:
Substances chimiques
Cell-Free Nucleic Acids
0
Culture Media, Conditioned
0
Lipopolysaccharides
0
Reactive Oxygen Species
0
Cannabidiol
19GBJ60SN5
MPO protein, human
EC 1.11.1.7
Peroxidase
EC 1.11.1.7
NADPH Oxidases
EC 1.6.3.-
neutrophil cytosolic factor 1
EC 1.6.3.1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Narodowe Centrum Nauki
ID : 2016/23/B/NZ7/02350
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