Hyperlipidemia does not affect development of elastase-induced abdominal aortic aneurysm in mice.


Journal

Atherosclerosis
ISSN: 1879-1484
Titre abrégé: Atherosclerosis
Pays: Ireland
ID NLM: 0242543

Informations de publication

Date de publication:
10 2020
Historique:
received: 18 05 2020
revised: 31 07 2020
accepted: 26 08 2020
pubmed: 20 9 2020
medline: 24 6 2021
entrez: 19 9 2020
Statut: ppublish

Résumé

Hyperlipidemia is a suggested risk factor for abdominal aortic aneurysm (AAA). However, whether hyperlipidemia is causally involved in AAA progression remains elusive. Here, we tested the hypothesis that hyperlipidemia aggravates AAA formation in the widely used porcine pancreatic elastase (PPE) model of AAA in mice with varying levels of plasma lipids. Prior to PPE-surgery, 8-week-old male C57BL/6J mice (n = 32) received 1·10 At sacrifice, maximal intergroup plasma cholesterol and non-HDL/HDL ratio differences were >5-fold and >20-fold, respectively. AAA diameters expanded to 150% of baseline, but no intergroup differences were detected. This was verified in an independent experiment comparing 8-week-old male ApoE In summary, we find that lipid deposition in the aortic wall is a feature of PPE-induced AAA in mice as well as human AAA lesions. Despite, our data do not support the hypothesis that hyperlipidemia contributes to AAA progression.

Sections du résumé

BACKGROUND AND AIMS
Hyperlipidemia is a suggested risk factor for abdominal aortic aneurysm (AAA). However, whether hyperlipidemia is causally involved in AAA progression remains elusive. Here, we tested the hypothesis that hyperlipidemia aggravates AAA formation in the widely used porcine pancreatic elastase (PPE) model of AAA in mice with varying levels of plasma lipids.
METHODS
Prior to PPE-surgery, 8-week-old male C57BL/6J mice (n = 32) received 1·10
RESULTS
At sacrifice, maximal intergroup plasma cholesterol and non-HDL/HDL ratio differences were >5-fold and >20-fold, respectively. AAA diameters expanded to 150% of baseline, but no intergroup differences were detected. This was verified in an independent experiment comparing 8-week-old male ApoE
CONCLUSIONS
In summary, we find that lipid deposition in the aortic wall is a feature of PPE-induced AAA in mice as well as human AAA lesions. Despite, our data do not support the hypothesis that hyperlipidemia contributes to AAA progression.

Identifiants

pubmed: 32949946
pii: S0021-9150(20)30456-1
doi: 10.1016/j.atherosclerosis.2020.08.012
pii:
doi:

Substances chimiques

Pancreatic Elastase EC 3.4.21.36

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Pagination

73-83

Subventions

Organisme : NHLBI NIH HHS
ID : R56 HL135654
Pays : United States
Organisme : BLRD VA
ID : I01 BX002641
Pays : United States

Informations de copyright

Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.

Auteurs

Joscha Mulorz (J)

VA Palo Alto Health Care System, Palo Alto, CA, United States; Stanford University, Department of Cardiovascular Medicine, Stanford, CA, United States; Department of Vascular and Endovascular Surgery, University Hospital Düsseldorf, Heinrich-Heine-University, Düsseldorf, Germany. Electronic address: joscha.mulorz@med.uni-duesseldorf.de.

Joshua Michael Spin (JM)

VA Palo Alto Health Care System, Palo Alto, CA, United States; Stanford University, Department of Cardiovascular Medicine, Stanford, CA, United States.

Hans Christian Beck (HC)

Department of Clinical Biochemistry and Pharmacology, Odense University Hospital, Odense, Denmark.

My Linh Tha Thi (ML)

Centre for Individualized Medicine in Arterial Diseases, Odense University Hospital, Odense, Denmark.

Markus Udo Wagenhäuser (MU)

Department of Vascular and Endovascular Surgery, University Hospital Düsseldorf, Heinrich-Heine-University, Düsseldorf, Germany.

Lars Melholt Rasmussen (LM)

Department of Clinical Biochemistry and Pharmacology, Odense University Hospital, Odense, Denmark; Centre for Individualized Medicine in Arterial Diseases, Odense University Hospital, Odense, Denmark.

Jes Sanddal Lindholt (JS)

Centre for Individualized Medicine in Arterial Diseases, Odense University Hospital, Odense, Denmark; Department of Cardiothoracic and Vascular Surgery, Odense University Hospital, Odense, Denmark.

Philip Shih Chung Tsao (PSC)

VA Palo Alto Health Care System, Palo Alto, CA, United States; Stanford University, Department of Cardiovascular Medicine, Stanford, CA, United States.

Lasse Bach Steffensen (LB)

Stanford University, Department of Cardiovascular Medicine, Stanford, CA, United States; Department of Clinical Biochemistry and Pharmacology, Odense University Hospital, Odense, Denmark; Centre for Individualized Medicine in Arterial Diseases, Odense University Hospital, Odense, Denmark; Unit of Cardiovascular and Renal Research, Department of Molecular Medicine, University of Southern Denmark, Odense, Denmark.

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Classifications MeSH