Architecture of the centriole cartwheel-containing region revealed by cryo-electron tomography.
in situ
cartwheel
centriole
cryo-electron tomography
cryo-focused ion beam milling
Journal
The EMBO journal
ISSN: 1460-2075
Titre abrégé: EMBO J
Pays: England
ID NLM: 8208664
Informations de publication
Date de publication:
16 11 2020
16 11 2020
Historique:
received:
15
07
2020
revised:
20
08
2020
accepted:
24
08
2020
pubmed:
22
9
2020
medline:
15
4
2021
entrez:
21
9
2020
Statut:
ppublish
Résumé
Centrioles are evolutionarily conserved barrels of microtubule triplets that form the core of the centrosome and the base of the cilium. While the crucial role of the proximal region in centriole biogenesis has been well documented, its native architecture and evolutionary conservation remain relatively unexplored. Here, using cryo-electron tomography of centrioles from four evolutionarily distant species, we report on the architectural diversity of the centriole's proximal cartwheel-bearing region. Our work reveals that the cartwheel central hub is constructed from a stack of paired rings with cartwheel inner densities inside. In both Paramecium and Chlamydomonas, the repeating structural unit of the cartwheel has a periodicity of 25 nm and consists of three ring pairs, with 6 radial spokes emanating and merging into a single bundle that connects to the microtubule triplet via the D2-rod and the pinhead. Finally, we identified that the cartwheel is indirectly connected to the A-C linker through the triplet base structure extending from the pinhead. Together, our work provides unprecedented evolutionary insights into the architecture of the centriole proximal region, which underlies centriole biogenesis.
Identifiants
pubmed: 32954513
doi: 10.15252/embj.2020106246
pmc: PMC7667884
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e106246Subventions
Organisme : EC|H2020|H2020 Priority Excellent Science|H2020 European Research Council ERC
ID : StG 715289
Organisme : Swiss National Science Foundation
ID : PP00P3_187198, PP00P3_157517
Pays : Switzerland
Informations de copyright
© 2020 The Authors. Published under the terms of the CC BY 4.0 license.
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