Novel nanoemulsion gel containing triple natural bio-actives combination of curcumin, thymoquinone, and resveratrol improves psoriasis therapy: in vitro and in vivo studies.
Anti-psoriatic assay
Curcumin
MTT assay
Nanoemulgel
Resveratrol
Thymoquinone
Journal
Drug delivery and translational research
ISSN: 2190-3948
Titre abrégé: Drug Deliv Transl Res
Pays: United States
ID NLM: 101540061
Informations de publication
Date de publication:
06 2021
06 2021
Historique:
accepted:
07
09
2020
pubmed:
24
9
2020
medline:
8
4
2022
entrez:
23
9
2020
Statut:
ppublish
Résumé
Curcumin, resveratrol, and thymoquinone are the potential natural bio-actives reported with good anti-psoriatic activity. However, poor aqueous solubility and limited skin permeation of these natural bio-actives hinder their effective delivery and potential therapeutic outcome. In this regard, current research work focuses on the design and optimization of nanoemulsion (NE) gel formulation for the concurrent delivery of these three drugs. The NE system is consisting of oleic acid as oil phase, Tween 20 as surfactant, and PEG 200 as co-surfactant. The optimized formulation exhibited the droplet size 76.20 ± 1.67 nm, PDI of 0.12 ± 0.05, RI of 1.403 ± 0.007, and viscosity of 137.9 ± 4.07 mp. Carbopol 940 (0.5% w/v) was used as the gelling agent to prepare the NE gel which exhibited a good texture profile. The optimized formulation exhibited a higher % of growth inhibition on A-431 cells and demonstrated good anti-angiogenic activity in the HET-CAM test. Finally, in vivo studies in Balb/c mice model showed improved anti-psoriatic conditions which indicated that the triple natural bio-actives combination in nanoemulgel formulation is effective in the management of psoriasis.
Identifiants
pubmed: 32965640
doi: 10.1007/s13346-020-00852-y
pii: 10.1007/s13346-020-00852-y
doi:
Substances chimiques
Benzoquinones
0
Emulsions
0
Curcumin
IT942ZTH98
thymoquinone
O60IE26NUF
Resveratrol
Q369O8926L
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1245-1260Références
Menter A, Korman NJ, Elmets CA, Feldman SR, Gelfand JM, Gordon KB, et al. Guidelines of care for the management of psoriasis and psoriatic arthritis: section 3. Guidelines of care for the management and treatment of psoriasis with topical therapies. J Am Acad Dermatol. 2009;60:643–59.
pubmed: 19217694
doi: 10.1016/j.jaad.2008.12.032
pmcid: 19217694
Abdelbary AA, AbouGhaly MH. Design and optimization of topical methotrexate loaded niosomes for enhanced management of psoriasis: application of Box–Behnken design, in-vitro evaluation and in-vivo skin deposition study. Int J Pharm. 2015;485:235–43.
pubmed: 25773359
doi: 10.1016/j.ijpharm.2015.03.020
pmcid: 25773359
Raychaudhuri SK, Maverakis E, Raychaudhuri SP. Diagnosis and classification of psoriasis. Autoimmun Rev. 2014;13:490–5.
pubmed: 24434359
doi: 10.1016/j.autrev.2014.01.008
pmcid: 24434359
Bailey EE, Ference EH, Alikhan A, Hession MT, Armstrong AW. Combination treatments for psoriasis: a systematic review and meta-analysis. Arch Dermatol. 2012;148:511–22.
pubmed: 22184718
doi: 10.1001/archdermatol.2011.1916
pmcid: 22184718
Kaur A, Katiyar SS, Kushwah V, Jain S. Nanoemulsion loaded gel for topical co-delivery of clobitasol propionate and calcipotriol in psoriasis. Nanomedicine. 2017;13:1473–82.
pubmed: 28259803
doi: 10.1016/j.nano.2017.02.009
pmcid: 28259803
Badıllı U, Şen T, Tarımcı N. Microparticulate based topical delivery system of clobetasol propionate. AAPS PharmSciTech. 2011;12:949–57.
pubmed: 21748539
pmcid: 3167258
doi: 10.1208/s12249-011-9661-7
Ali A, Ali S, Aqil M, Imam SS, Ahad A, Khan AQ. Thymoquinone loaded dermal lipid nano particles: Box Behnken design optimization to preclinical psoriasis assessment. J Drug Delivery Sci Technol. 2019;52:713–21.
doi: 10.1016/j.jddst.2019.05.041
Akhtar M, Imam SS, Afroz Ahmad M, Najmi AK, Mujeeb M, Aqil M. Neuroprotective study of Nigella sativa-loaded oral provesicular lipid formulation: in vitro and ex vivo study. Drug Deliv. 2014;21:487–94.
pubmed: 24601853
doi: 10.3109/10717544.2014.886640
pmcid: 24601853
Babazadeh B, Sadeghnia HR, Kapurchal ES, Parsaee H, Nasri S, Tayarani-Najaran Z. Protective effect of Nigella sativa and thymoquinone on serum/glucose deprivation-induced DNA damage in PC12 cells. Avicenna J Phytomed. 2012;2:125.
pubmed: 25050241
pmcid: 4075672
Sayeed S, Imam SS, Najmi AK, Aqil M, Akhtar M. Nonionic surfactant based thymoquinone loaded nanoproniosomal formulation: in vitro physicochemical evaluation and in vivo hepatoprotective efficacy. Drug Dev Ind Pharm. 2017;43:1413–20.
pubmed: 28402205
doi: 10.1080/03639045.2017.1318903
Jain A, Pooladanda V, Bulbake U, Doppalapudi S, Rafeeqi TA, Godugu C, et al. Liposphere mediated topical delivery of thymoquinone in the treatment of psoriasis. Nanomedicine. 2017;13:2251–62.
pubmed: 28647592
doi: 10.1016/j.nano.2017.06.009
Löbenberg R, Amidon GL. Modern bioavailability, bioequivalence and biopharmaceutics classification system. New scientific approaches to international regulatory standards. Eur J Pharm Biopharm. 2000;50:3–12.
pubmed: 10840189
doi: 10.1016/S0939-6411(00)00091-6
Kjær TN, Thorsen K, Jessen N, Stenderup K, Pedersen SB. Resveratrol ameliorates imiquimod-induced psoriasis-like skin inflammation in mice. PLoS One. 2015;10:e0126599.
pubmed: 25965695
pmcid: 4428792
doi: 10.1371/journal.pone.0126599
Shehzad A, Wahid F, Lee YS. Curcumin in cancer chemoprevention: molecular targets, pharmacokinetics, bioavailability, and clinical trials. Arch Pharm. 2010;343:489–99.
doi: 10.1002/ardp.200900319
Barygina V, Becatti M, Soldi G, Prignano F, Lotti T, Nassi P, et al. Altered redox status in the blood of psoriatic patients: involvement of NADPH oxidase and role of anti-TNF-α therapy. Redox Rep. 2013;18:100–6.
pubmed: 23601139
pmcid: 6837649
doi: 10.1179/1351000213Y.0000000045
Di Nardo V, Gianfaldoni S, Tchernev G, Wollina U, Barygina V, Lotti J, et al. Use of curcumin in psoriasis. Open Access Maced J Med Sci. 2018;6:218–20.
pubmed: 29484027
pmcid: 5816303
doi: 10.3889/oamjms.2018.055
Pradhan M, Singh D, Singh MR. Novel colloidal carriers for psoriasis: current issues, mechanistic insight and novel delivery approaches. J Control Release. 2013;170:380–95.
pubmed: 23770117
doi: 10.1016/j.jconrel.2013.05.020
Acharya U, Rajarajan S, Acharya R, Acharya R, Chaudhary G, Ghimire S. Formulation, and evaluation of nanoemulsion based system for transdermal delivery of the antipsoriatic drug. World J Pharm Pharm Sci. 2017;6:732–48.
Mou D, Chen H, Du D, Mao C, Wan J, Xu H, et al. Hydrogel-thickened nanoemulsion system for topical delivery of lipophilic drugs. Int J Pharm. 2008;353:270–6.
pubmed: 18215479
doi: 10.1016/j.ijpharm.2007.11.051
Shaikh GH. Alternative medicine for psoriasis–natural herbal ayurvedic treatment—a review. J Curr Res Ayurvedic Pharm Sci. 2012;3.
Mahtab A, Anwar M, Mallick N, Naz Z, Jain GK, Ahmad FJ. Transungual delivery of ketoconazole nanoemulgel for the effective management of onychomycosis. AAPS PharmSciTech. 2016;17:1477–90.
pubmed: 26857516
doi: 10.1208/s12249-016-0488-0
Akhter S, Jain GK, Ahmad FJ, Khar RK, Jain N, Khan ZI, et al. Investigation of nanoemulsion system for transdermal delivery of domperidone: ex-vivo and in vivo studies. Curr Nanosci. 2008;4:381–90.
doi: 10.2174/157341308786306071
Haider MF, Khan S, Gaba B, Alam T, Baboota S, Ali J, et al. Optimization of rivastigmine nanoemulsion for enhanced brain delivery: in-vivo and toxicity evaluation. J Mol Liq. 2018;255:384–96.
doi: 10.1016/j.molliq.2018.01.123
Azeem A, Rizwan M, Ahmad FJ, Iqbal Z, Khar RK, Aqil M, et al. Nanoemulsion components screening and selection: a technical note. AAPS PharmSciTech. 2009;10:69–76.
pubmed: 19148761
pmcid: 2663668
doi: 10.1208/s12249-008-9178-x
Gaba B, Khan T, Haider MF, Alam T, Baboota S, Parvez S, et al. Vitamin E loaded naringenin nanoemulsion via intranasal delivery for the management of oxidative stress in a 6-OHDA Parkinson’s disease model. Biomed Res Int. 2019;2019:1–20.
doi: 10.1155/2019/2382563
Khatoon K, Rizwanullah M, Amin S, Mir SR, Akhter S. Cilnidipine loaded transfersomes for transdermal application: formulation optimization, in-vitro and in-vivo study. J Drug Delivery Sci Technol. Elsevier. 2019;54:101303.
doi: 10.1016/j.jddst.2019.101303
Sahu S, Katiyar SS, Kushwah V, Jain S. Active natural oil-based nanoemulsion containing tacrolimus for synergistic antipsoriatic efficacy. Nanomedicine. 2018;13:1985–98.
pubmed: 30188761
doi: 10.2217/nnm-2018-0135
Baboota S, Alam MS, Sharma S, Sahni JK, Kumar A, Ali J. Nanocarrier-based hydrogel of betamethasone dipropionate and salicylic acid for treatment of psoriasis. Int J Pharm Investig. 2011;1:139–47.
pubmed: 23071936
pmcid: 3465136
doi: 10.4103/2230-973X.85963
de Vargas BA, Bidone J, Oliveira LK, Koester LS, Bassani VL, Teixeira HF. Development of topical hydrogels containing genistein-loaded nanoemulsions. J Biomed Nanotechnol. American Scientific Publishers. 2012;8:330–6.
pubmed: 22515085
doi: 10.1166/jbn.2012.1386
Hussain A, Samad A, Nazish I, Ahmed FJ. Nanocarrier-based topical drug delivery for an antifungal drug. Drug Dev Ind Pharm. 2014;40:527–41.
pubmed: 23627443
doi: 10.3109/03639045.2013.771647
pmcid: 23627443
Kota K, Sharma S, Ragavendhra P. Study of antiangiogenic activity of “aqueous extract of Nigella sativa seeds” in chick chorioallantoic membrane (CAM) model. Int J Adv Med. 2018;5:895.
doi: 10.18203/2349-3933.ijam20182944
Sun J, Zhao Y, Hu J. Curcumin inhibits imiquimod-induced psoriasis-like inflammation by inhibiting IL-1beta and IL-6 production in mice. PLoS One. 2013;8:e67078.
pubmed: 23825622
pmcid: 3692410
doi: 10.1371/journal.pone.0067078
Draize JH. Methods for the study of irritation and toxicity of substances applied topically to the skin and mucous membranes. J Pharmacol Exp Ther. 1944;82:377–90.
Touitou E, Godin B, Karl Y, Bujanover S, Becker Y. Oleic acid, a skin penetration enhancer, affects Langerhans cells and corneocytes. J Control Release. 2002;80:1–7.
pubmed: 11943383
doi: 10.1016/S0168-3659(02)00004-4
pmcid: 11943383
Sood S, Jain K, Gowthamarajan K. Optimization of curcumin nanoemulsion for intranasal delivery using design of experiment and its toxicity assessment. Colloids Surf B: Biointerfaces. 2014;113:330–7.
pubmed: 24121076
doi: 10.1016/j.colsurfb.2013.09.030
pmcid: 24121076
El Maghraby GM. Transdermal delivery of hydrocortisone from eucalyptus oil microemulsion: effects of cosurfactants. Int J Pharm. 2008;355:285–92.
pubmed: 18243604
doi: 10.1016/j.ijpharm.2007.12.022
pmcid: 18243604
Anarjan N, Tan C. Effects of selected polysorbate and sucrose ester emulsifiers on the physicochemical properties of astaxanthin nanodispersions. Molecules. 2013;18:768–77.
pubmed: 23303336
pmcid: 6270025
doi: 10.3390/molecules18010768
Heuschkel S, Goebel A, Neubert RH. Microemulsions—modern colloidal carrier for dermal and transdermal drug delivery. J Pharm Sci. 2008;97:603–31.
pubmed: 17696162
doi: 10.1002/jps.20995
Ahmed S, Gull A, Alam M, Aqil M, Sultana Y. Ultrasonically tailored, chemically engineered and “QbD” enabled fabrication of agomelatine nanoemulsion; optimization, characterization, ex-vivo permeation and stability study. Ultrason Sonochem. 2018;41:213–26.
pubmed: 29137746
doi: 10.1016/j.ultsonch.2017.09.042
Wennerström H, Olsson U. Microemulsions as model systems. C R Chim. 2009;12:4–17.
doi: 10.1016/j.crci.2008.08.011
Lawrence MJ, Rees GD. Microemulsion-based media as novel drug delivery systems. Adv Drug Deliv Rev. 2000;45:89–121.
pubmed: 11104900
doi: 10.1016/S0169-409X(00)00103-4
Baboota S, Shakeel F, Ahuja A, Ali J, Shafiq S. Design, development and evaluation of novel nanoemulsion formulations for transdermal potential of celecoxib. Acta Pharma. 2007;57:315–32.
doi: 10.2478/v10007-007-0025-5
Belhaj N, Dupuis F, Arab-Tehrany E, Denis FM, Paris C, Lartaud I, et al. Formulation, characterization and pharmacokinetic studies of coenzyme Q10 PUFA’s nanoemulsions. Eur J Pharm Sci. 2012;47:305–12.
pubmed: 22732255
doi: 10.1016/j.ejps.2012.06.008
Tuğcu-Demiröz F. Vaginal delivery of benzydamine hydrochloride through liposomes dispersed in mucoadhesive gels. Chem Pharm Bull. The Pharmaceutical Society of Japan. 2017:c17–00133.
Jose A, Labala S, Ninave KM, Gade SK, Venuganti VVK. Effective skin cancer treatment by topical co-delivery of curcumin and STAT3 siRNA using cationic liposomes. AAPS PharmSciTech. 2018;19:166–75.
pubmed: 28639178
doi: 10.1208/s12249-017-0833-y
Zhai X, Ding J, Tang Z, Li J, Li Y, Yan Y, et al. Effects of resveratrol on the proliferation, apoptosis and telomerase ability of human A431 epidermoid carcinoma cells. Oncol Lett. Spandidos Publications. 2016;11:3015–8.
pubmed: 27123055
pmcid: 4840784
doi: 10.3892/ol.2016.4351
Park JE, Kim D-H, Ha E, Choi SM, Choi J-S, Chun K-S, et al. Thymoquinone induces apoptosis of human epidermoid carcinoma A431 cells through ROS-mediated suppression of STAT3. Chem Biol Interact. Elsevier. 2019;312:108799.
pubmed: 31433961
doi: 10.1016/j.cbi.2019.108799
pmcid: 31433961
Agarwal S, Srivastava R, Mishra N. An overview of therapeutic potential of thymoquinone. Int J Pharm Sci Res. 2019;10(8):3532–3539.
Gambini J, Inglés M, Olaso G, Lopez-Grueso R, Bonet-Costa V, Gimeno-Mallench L, et al. Properties of resveratrol: in vitro and in vivo studies about metabolism, bioavailability, and biological effects in animal models and humans. Oxidative Med Cell Longev. Hindawi. 2015:2015.
Priyadarsini KI. The chemistry of curcumin: from extraction to therapeutic agent. Molecules. Multidisciplinary Digital Publishing Institute. 2014;19:20091–112.
pubmed: 25470276
pmcid: 6270789
doi: 10.3390/molecules191220091
Alvarez-Román R, Naik A, Kalia Y, Guy RH, Fessi H. Skin penetration and distribution of polymeric nanoparticles. J Control Release. 2004;99:53–62.
pubmed: 15342180
doi: 10.1016/j.jconrel.2004.06.015
Sankar L, Arumugam D, Sudha Boj PP. Expression of angiogenic factors in psoriasis vulgaris. J Clin Diagn Res. JCDR Research & Publications Private Limited. 2017;11:EC23.
pubmed: 28511390
pmcid: 5427316
Panonnummal R, Jayakumar R, Anjaneyan G, Sabitha M. In vivo anti-psoriatic activity, biodistribution, sub-acute and sub-chronic toxicity studies of orally administered methotrexate loaded chitin nanogel in comparison with methotrexate tablet. Int J Biol Macromol. Elsevier. 2018;110:259–68.
pubmed: 29355632
doi: 10.1016/j.ijbiomac.2018.01.036