3-photon microscopy of myelin in mouse digital skin excited at the 1700-nm window.
1700-nm window
3-photon microscopy
digital skin
myelin
Journal
Journal of biophotonics
ISSN: 1864-0648
Titre abrégé: J Biophotonics
Pays: Germany
ID NLM: 101318567
Informations de publication
Date de publication:
12 2020
12 2020
Historique:
received:
09
08
2020
revised:
20
09
2020
accepted:
22
09
2020
pubmed:
25
9
2020
medline:
24
6
2021
entrez:
24
9
2020
Statut:
ppublish
Résumé
Myelin is a key component of the peripheral nervous system, whose structure anomaly in the digital skin is implicated in neuropathy. Here we demonstrate an in vivo labeling and imaging technique, capable of visualizing myelin sheaths deep in the mouse digital skin. Through material characterization, we verify that 3-photon fluorescence (3PF) can be generated from a commonly used dye- FluoroMyelin Red for labeling myelin, excited at the 1700-nm window. Topical injection of FluoroMyelin Red in the mouse digit leads to bright labeling of myelin sheaths. Harnessing the deep-penetration capability of 3-photon microscopy excited at the 1700-nm window, we demonstrate that 3PF imaging of FluoroMyelin Red-labeled myelin sheaths in the mouse digit in vivo can be achieved to a depth 340 μm below the skin surface, revealing both branching bundle of and individual myelin sheaths.
Identifiants
pubmed: 32969170
doi: 10.1002/jbio.202000321
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e202000321Informations de copyright
© 2020 Wiley-VCH GmbH.
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