Sotolon is a natural virulence mitigating agent in Serratia marcescens.
Anti-Bacterial Agents
/ pharmacology
Biofilms
/ drug effects
Cross Infection
/ drug therapy
Enzyme Activation
/ drug effects
Furans
/ pharmacology
Gene Expression Regulation, Bacterial
/ drug effects
Humans
Peptide Hydrolases
/ metabolism
Prodigiosin
/ metabolism
Quorum Sensing
/ drug effects
Serratia Infections
/ drug therapy
Serratia marcescens
/ drug effects
Virulence Factors
/ genetics
Anti-virulence
Antibiotic resistance
Serratia marcescens
Sotolon
Journal
Archives of microbiology
ISSN: 1432-072X
Titre abrégé: Arch Microbiol
Pays: Germany
ID NLM: 0410427
Informations de publication
Date de publication:
Mar 2021
Mar 2021
Historique:
received:
31
03
2020
accepted:
12
09
2020
revised:
25
08
2020
pubmed:
25
9
2020
medline:
6
3
2021
entrez:
24
9
2020
Statut:
ppublish
Résumé
Serratia marcescens is an emerging opportunistic bacterium that can cause healthcare-associated infections. The high rate of multidrug resistance and the ability to produce a set of virulence factors, by which it can produce infectious diseases makes it urgent to find an alternative approach to the treatment of such infections. Disarming of virulence by targeting of quorum sensing (QS) as the regulating mechanism of virulence is a promising approach that has no effect on bacterial growth that is considered a key factor in emergence of resistance. This study was designed to investigate the ability of sub-inhibitory concentrations (sub-MICs) of sotolon to attenuate virulence of a clinical isolate of S. marcescens. Sotolon at 25 and 50 μg/ml inhibited 35.2 and 47.5% of biofilm formation, respectively. The inhibition of swimming motility were 41.4 and 69.3%, while that of swarming motility were 77.6 and 86.8% at 25 and 50 µg/ml, respectively. Moreover, sotolon reduced prodigiosin production by 76.6 and 87.6% at concentrations of 25 and 50 µg/ml, respectively. Protease activity was reduced by 25 µg/ml of sotolon by 54.8% and was completely blocked at 50 µg/ml. The relative expression of genes regulating virulence factors decreased by 40% for fimA, 29% for fimC, 59% for flhC, 57% for flhD, 39% for bsmB, 37% for rssB, 49% for rsmA, 54% for pigP, and 62% for shlA gene in the presence of 50 µg/ml sotolon. In conclusion, sotolon is an anti-virulence agent that could be used for the treatment of S.marcescens hospital-acquired infections.
Identifiants
pubmed: 32970221
doi: 10.1007/s00203-020-02039-y
pii: 10.1007/s00203-020-02039-y
doi:
Substances chimiques
Anti-Bacterial Agents
0
Furans
0
Virulence Factors
0
4,5-dimethyl-3-hydroxy-2(5H)-furanone
28664-35-9
Peptide Hydrolases
EC 3.4.-
Prodigiosin
OL369FU7CJ
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
533-541Références
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