Renilla Luciferase Reporter Assay to Study 3'-UTR-Driven Posttranscriptional Regulation of OPRM1.
3' Untranslated Regions
/ genetics
Animals
Gene Expression
/ genetics
Gene Expression Profiling
/ methods
Gene Expression Regulation
/ genetics
Genes, Reporter
/ genetics
Humans
Luciferases, Renilla
/ metabolism
RNA Processing, Post-Transcriptional
/ genetics
RNA, Messenger
/ genetics
Receptors, Opioid, mu
/ genetics
3′-UTR
Gene reporter assay
Mu opioid receptor
Posttranscriptional regulation
Renilla luciferase
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
25
9
2020
pubmed:
26
9
2020
medline:
23
3
2021
Statut:
ppublish
Résumé
MOR expression levels at a specific cell type or tissue significantly contribute to its role in pain transmission and in other responses involving opioid receptors. Therefore, molecular processes regulating MOR levels have gained more and more interest. Recently, posttranscriptional regulation mechanisms have been shown to play a relevant role in influencing MOR expression levels, with polymorphisms and mutations within OPRM1 3'-UTR region impacting the differential opioid-mediated response observed within individuals. Here we report a Renilla luciferase reporter assay format suitable for dissecting the contribution of different and distinct OPRM1 3'-UTR elements to MOR expression levels in a model of glial cells, both under basal conditions and following specific treatments.
Identifiants
pubmed: 32975785
doi: 10.1007/978-1-0716-0884-5_2
doi:
Substances chimiques
3' Untranslated Regions
0
OPRM1 protein, human
0
RNA, Messenger
0
Receptors, Opioid, mu
0
Luciferases, Renilla
EC 1.13.12.5
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM