The microRNAs miR-302d and miR-93 inhibit TGFB-mediated EMT and VEGFA secretion from ARPE-19 cells.
Blotting, Western
Cell Line
Enzyme-Linked Immunosorbent Assay
Epithelial-Mesenchymal Transition
/ physiology
Gene Expression Regulation
Humans
MicroRNAs
/ biosynthesis
RNA
/ genetics
Retinal Pigment Epithelium
/ metabolism
Transforming Growth Factor beta
/ biosynthesis
Vascular Endothelial Growth Factor A
/ metabolism
Wet Macular Degeneration
/ genetics
AMD
ARPE-19
EMT
PVR
RPE
TGFB
VEGFA
miR-302
miR-93
microRNA
Journal
Experimental eye research
ISSN: 1096-0007
Titre abrégé: Exp Eye Res
Pays: England
ID NLM: 0370707
Informations de publication
Date de publication:
12 2020
12 2020
Historique:
received:
07
02
2020
revised:
31
07
2020
accepted:
18
09
2020
pubmed:
28
9
2020
medline:
2
3
2021
entrez:
27
9
2020
Statut:
ppublish
Résumé
The transforming growth factor-beta (TGFB) plays an essential role in the pathogenesis of some ophthalmologic diseases, including neovascular age-related macular degeneration (nAMD) and proliferative vitreoretinopathy (PVR). TGFB activates the transcription factors SMAD2 and SMAD3 via the TGFB receptor, which together activate several genes, including VEGFA. TGFB treated ARPE-19 cells show an increased proliferation rate and undergo epithelial to mesenchymal transition (EMT). Since microRNAs (miRNAs) are capable of inhibiting the translation of multiple genes, we screened for miRNAs that regulate the TGFB signalling pathways at multiple levels. In this study, we focused on two miRNAs, miR-302d and miR-93, which inhibit TGFB signalling pathway and therefore TGFB-induced EMT transition as well as VEGFA secretion from ARPE-19 cells. Furthermore, we could show that both miRNAs can retransform TGFB-stimulated mesenchymal ARPE-19 cells towards the morphological epithelial-like state. Taken together, transient overexpression of these miRNAs in RPE cells might be a promising approach for further translational strategies.
Identifiants
pubmed: 32980316
pii: S0014-4835(20)30516-9
doi: 10.1016/j.exer.2020.108258
pii:
doi:
Substances chimiques
MIRN93 microRNA, human
0
MicroRNAs
0
Transforming Growth Factor beta
0
Vascular Endothelial Growth Factor A
0
RNA
63231-63-0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
108258Informations de copyright
Copyright © 2020 Elsevier Ltd. All rights reserved.