Beauvericin alters the expression of genes coding for key proteins of the mitochondrial chain in ovine cumulus-oocyte complexes.
Beauvericin
Cumulus cells
Gene expression
Mitochondria
Oocyte
Ovine
Journal
Mycotoxin research
ISSN: 1867-1632
Titre abrégé: Mycotoxin Res
Pays: Germany
ID NLM: 8807334
Informations de publication
Date de publication:
Feb 2021
Feb 2021
Historique:
received:
10
02
2020
accepted:
15
09
2020
revised:
09
09
2020
pubmed:
28
9
2020
medline:
6
8
2021
entrez:
27
9
2020
Statut:
ppublish
Résumé
Beauvericin (BEA) is a member of the enniatin family of mycotoxins which has received increasing interest because of frequent occurrence in food and feed. By its ionophoric properties, BEA is able to alter membrane ion permeability uncoupling oxidative phosphorylation. It was also shown to alter oocyte mitochondrial function. In this study, the effects of BEA at 0.5, 1, ,3 and 5 μmol/L on expression of genes coding for key proteins of the mitochondrial chain in ovine oocytes and cumulus cells were evaluated at different time points of in vitro maturation (IVM), germinal vesicle (GV; t = 0), metaphase I (MI; t = 7 h), and metaphase II (MII; t = 24 h). The expression of nuclear (TFAM, NDUFA12, UQCRH, COX4, ATP5O) and mitochondrial (ND1, COX1, COX2, ATP6, ATP8) genes coding for proteins of Complexes I, III, IV, and V was analyzed by qRT-PCR. After BEA exposure, perturbed expression of all genes was observed in cumulus cells and in oocytes at the MI stage (7 h IVM). Expression of ND1, UQCRH, COX4 and ATP5O was downregulated in cumulus cells and upregulated in oocytes starting from 0.5 μmol/L BEA. Expression of TFAM, NDUFA12, COX1, COX2, ATP6, and ATP8 was upregulated starting from 1 μmol/L in cumulus cells and from 3 μmol/L in oocytes. Cumulus cells and oocytes displayed different gene expression patterns upon BEA exposure. The downregulation in cumulus cells of four genes coding for proteins of mitochondrial complexes could represent a major toxic event induced by BEA on the cumulus-oocyte complex which may result in mitochondrial functional alteration.
Identifiants
pubmed: 32981022
doi: 10.1007/s12550-020-00409-5
pii: 10.1007/s12550-020-00409-5
doi:
Substances chimiques
Depsipeptides
0
Mitochondrial Proteins
0
Mycotoxins
0
beauvericin
26S048LS2R
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1-9Références
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