Alternative conformations and motions adopted by 30S ribosomal subunits visualized by cryo-electron microscopy.
30S subunit
cryo-electron microscopy
ribosome structure
Journal
RNA (New York, N.Y.)
ISSN: 1469-9001
Titre abrégé: RNA
Pays: United States
ID NLM: 9509184
Informations de publication
Date de publication:
Dec 2020
Dec 2020
Historique:
received:
08
04
2020
accepted:
22
09
2020
pubmed:
30
9
2020
medline:
26
1
2021
entrez:
29
9
2020
Statut:
ppublish
Résumé
It is only after recent advances in cryo-electron microscopy that it is now possible to describe at high-resolution structures of large macromolecules that do not crystalize. Purified 30S subunits interconvert between an "active" and "inactive" conformation. The active conformation was described by crystallography in the early 2000s, but the structure of the inactive form at high resolution remains unsolved. Here we used cryo-electron microscopy to obtain the structure of the inactive conformation of the 30S subunit to 3.6 Å resolution and study its motions. In the inactive conformation, an alternative base-pairing of three nucleotides causes the region of helix 44, forming the decoding center to adopt an unlatched conformation and the 3' end of the 16S rRNA positions similarly to the mRNA during translation. Incubation of inactive 30S subunits at 42°C reverts these structural changes. The air-water interface to which ribosome subunits are exposed during sample preparation also peel off some ribosomal proteins. Extended exposures to low magnesium concentrations make the ribosomal particles more susceptible to the air-water interface causing the unfolding of large rRNA structural domains. Overall, this study provides new insights about the conformational space explored by the 30S ribosomal subunit when the ribosomal particles are free in solution.
Identifiants
pubmed: 32989043
pii: rna.075846.120
doi: 10.1261/rna.075846.120
pmc: PMC7668263
doi:
Substances chimiques
RNA, Ribosomal, 16S
0
Ribosomal Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2017-2030Informations de copyright
© 2020 Jahagirdar et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society.
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