Biofilm removal from a simulated isthmus and lateral canal during syringe irrigation at various flow rates: a combined experimental and Computational Fluid Dynamics approach.
biofilm
isthmus
lateral canal
sodium hypochlorite
velocity
Journal
International endodontic journal
ISSN: 1365-2591
Titre abrégé: Int Endod J
Pays: England
ID NLM: 8004996
Informations de publication
Date de publication:
Mar 2021
Mar 2021
Historique:
received:
20
12
2019
revised:
21
09
2020
accepted:
22
09
2020
pubmed:
30
9
2020
medline:
16
2
2021
entrez:
29
9
2020
Statut:
ppublish
Résumé
(i) To quantify biofilm removal from a simulated isthmus and a lateral canal in an artificial root canal system during syringe irrigation with NaOCl at different concentrations and delivered at various flow rates (ii) to examine whether biofilm removal is further improved by a final high-flow-rate rinse with an inert irrigant following irrigation with NaOCl. (iii) to simulate the irrigant flow in these areas using a computer model (iv) to examine whether the irrigant velocity calculated by the computer model is correlated to biofilm removal. Ninety-six artificial root canals with either a simulated isthmus or lateral canal were used. A dual-species in vitro biofilm was formed in these areas using a Constant Depth Film Fermenter. NaOCl at various concentrations (2, 5 and 10%) or adhesion buffer (control) was delivered for 30 s by a syringe and an open-ended needle at 0.033, 0.083, or 0.166 mL s The flow rate during phase 1 and additional irrigation during phase 2 had a significant effect on the percentage of the remaining biofilm in the isthmus (P = 0.004 and P < 0.001). Additional irrigation during phase 2 also affected the remaining biofilm in the lateral canal significantly (P ≤ 0.007) but only when preceded by irrigation at medium or high flow rate during phase 1. The effect of NaOCl concentration was not significant (P > 0.05). Irrigant velocity in the isthmus and lateral canal increased with increasing flow rate and it was substantially correlated to biofilm removal from those areas. The irrigant flow rate affected biofilm removal in vitro more than NaOCl concentration. Irrigant velocity predicted by the computer model corresponded with the pattern of biofilm removal from the simulated isthmus and lateral canal.
Identifiants
pubmed: 32990985
doi: 10.1111/iej.13420
pmc: PMC7894139
doi:
Substances chimiques
Root Canal Irrigants
0
Sodium Hypochlorite
DY38VHM5OD
Types de publication
Journal Article
Langues
eng
Pagination
427-438Subventions
Organisme : Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
Organisme : Conselho Nacional de Desenvolvimento Científico e Tecnológico
Organisme : Abel Tasman Talent Program
Organisme : European Society of Endodontology
Organisme : the Abel Tasman Talent Program (ATTP) of the Graduate School of Medical Sciences of the University of Groningen
Organisme : CNPq
Informations de copyright
© 2020 The Authors. International Endodontic Journal published by John Wiley & Sons Ltd on behalf of British Endodontic Society.
Références
Int Endod J. 2014 Nov;47(11):1040-51
pubmed: 24397845
J Endod. 2002 Sep;28(9):634-7
pubmed: 12236305
Int Endod J. 2021 Mar;54(3):427-438
pubmed: 32990985
Int Endod J. 2010 Dec;43(12):1108-15
pubmed: 20812947
J Endod. 2010 Jan;36(1):1-15
pubmed: 20003929
Nat Methods. 2012 Jun 28;9(7):676-82
pubmed: 22743772
Appl Environ Microbiol. 2008 Sep;74(17):5511-5
pubmed: 18641154
Behav Res Methods. 2007 May;39(2):175-91
pubmed: 17695343
J Microbiol Methods. 2006 Sep;66(3):390-8
pubmed: 16487610
Int Endod J. 2000 Mar;33(2):126-31
pubmed: 11307453
Clin Oral Investig. 2010 Aug;14(4):403-9
pubmed: 19565279
PLoS One. 2013 May 27;8(5):e63750
pubmed: 23723995
Int Endod J. 2009 Feb;42(2):144-55
pubmed: 19134043
J Endod. 2010 May;36(5):875-9
pubmed: 20416437
Int Endod J. 2019 May;52(5):604-628
pubmed: 30488449
Int Endod J. 1982 Oct;15(4):187-96
pubmed: 6964523
J Endod. 2009 Apr;35(4):493-502
pubmed: 19345793
J Endod. 2013 May;39(5):712-8
pubmed: 23611398
Langmuir. 2014 Sep 2;30(34):10354-62
pubmed: 25117376
J Endod. 2006 May;32(5):389-98
pubmed: 16631834
Int Endod J. 2019 Apr;52(4):461-474
pubmed: 30303560
J Conserv Dent. 2016 May-Jun;19(3):199-206
pubmed: 27217630
J Endod. 2004 Aug;30(8):559-67
pubmed: 15273636
J Endod. 2012 Jan;38(1):37-40
pubmed: 22152617
Water Res. 2008 Apr;42(8-9):2102-10
pubmed: 18086485
Int Endod J. 2019 Aug;52(8):1182-1195
pubmed: 30807649
Int Endod J. 2010 May;43(5):393-403
pubmed: 20518932
Int Endod J. 2019 Dec;52(12):1773-1788
pubmed: 31389008
J Endod. 2015 Jun;41(6):884-9
pubmed: 25749254
J Dent Res. 2015 Aug;94(8):1027-34
pubmed: 26001706
Eur J Oral Sci. 2003 Dec;111(6):459-64
pubmed: 14632680