A distinctive pathway for triplet-triplet energy transfer photoprotection in fucoxanthin chlorophyll-binding proteins from Cyclotella meneghiniana.

Chlorophyll Binding Proteins / chemistry Electron Spin Resonance Spectroscopy Stramenopiles / chemistry
Carotenoid EPR FCP Fucoxanthin Light-harvesting complex ODMR TTET Triplet state

Journal

Biochimica et biophysica acta. Bioenergetics
ISSN: 1879-2650
Titre abrégé: Biochim Biophys Acta Bioenerg
Pays: Netherlands
ID NLM: 101731706

Informations de publication

Date de publication:
01 01 2021
Historique:
received: 25 06 2020
revised: 08 09 2020
accepted: 10 09 2020
pubmed: 30 9 2020
medline: 14 4 2021
entrez: 29 9 2020
Statut: ppublish

Résumé

Fucoxanthin chlorophyll-binding proteins (FCPs) are the major light-harvesting complexes of diatoms. In this work, FCPs isolated from Cyclotella meneghiniana have been studied by means of optically detected magnetic resonance (ODMR) and time-resolved electron paramagnetic resonance (TR-EPR), with the aim to characterize the photoprotective mechanism based on triplet-triplet energy transfer (TTET). The spectroscopic properties of the chromophores carrying the triplet state have been interpreted on the basis of a delved analysis of the recently solved crystallographic structures of FCP. The results point toward a photoprotective role for two fucoxanthin molecules exposed to the exterior of the FCP monomers. This shows that FCP has adopted a structural strategy different from that of related light-harvesting complexes from plants and other microalgae, in which the photoprotective role is carried out by two highly conserved carotenoids in the interior of the complex.

Identifiants

DOI: 10.1016/j.bbabio.2020.148310 PMID: 32991847
pubmed: 32991847
pii: S0005-2728(20)30160-2
doi: 10.1016/j.bbabio.2020.148310
pii:
doi:

Substances chimiques

Chlorophyll Binding Proteins 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

148310

Informations de copyright

Copyright © 2020 Elsevier B.V. All rights reserved.

Auteurs

Alessandro Agostini (A)

Department of Chemical Sciences, University of Padova, via Marzolo 1, 35131 Padova, Italy. Electronic address: alessandro.agostini.1@unipd.it.

Claudia Büchel (C)

Institute of Molecular Biosciences, Goethe University Frankfurt, Max-von-Laue Str. 9, 60438 Frankfurt, Germany.

Marilena Di Valentin (M)

Department of Chemical Sciences, University of Padova, via Marzolo 1, 35131 Padova, Italy.

Donatella Carbonera (D)

Department of Chemical Sciences, University of Padova, via Marzolo 1, 35131 Padova, Italy. Electronic address: donatella.carbonera@unipd.it.

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines végétales › Protéines chloroplastiques › Protéines de la membrane thylakoïdale › Complexe protéique du centre réactionnel de la photosynthèse › Complexes collecteurs de lumière › Protéines liant la chlorophylle › A distinctive pathway for triplet-triplet...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Analyse spectrale › Spectroscopie par résonance magnétique › Spectroscopie de résonance de spin électronique › A distinctive pathway for triplet-triplet...
questionsmedicales.fr › Eucaryotes › Straménopiles › A distinctive pathway for triplet-triplet...