Cholinergic axons regulate type I acini in salivary glands of Ixodes ricinus and Ixodes scapularis ticks.
Acinar Cells
/ metabolism
Animals
Axons
/ metabolism
Central Nervous System
/ metabolism
Choline O-Acetyltransferase
/ genetics
Cholinergic Agents
/ metabolism
Cholinergic Neurons
/ metabolism
Ixodes
/ metabolism
Neurons
/ metabolism
RNA, Messenger
/ metabolism
Salivary Glands
/ metabolism
Signal Transduction
/ genetics
Vesicular Acetylcholine Transport Proteins
/ genetics
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
29 09 2020
29 09 2020
Historique:
received:
11
02
2020
accepted:
10
09
2020
entrez:
30
9
2020
pubmed:
1
10
2020
medline:
26
1
2021
Statut:
epublish
Résumé
Regulatory factors controlling tick salivary glands (SGs) are direct upstream neural signaling pathways arising from the tick's central nervous system. Here we investigated the cholinergic signaling pathway in the SG of two hard tick species. We reconstructed the organization of the cholinergic gene locus, and then used in situ hybridization to localize mRNA encoding choline acetyltransferase (ChAT) and vesicular acetylcholine transporter (VAChT) in specific neural cells in the Ixodes synganglion. Immunohistochemical staining revealed that cholinergic axonal projections exclusively reached type I acini in the SG of both Ixodes species. In type I acini, the rich network of cholinergic axons terminate within the basolateral infoldings of the lamellate cells. We also characterized two types (A and B) of muscarinic acetylcholine receptors (mAChRs), which were expressed in Ixodes SG. We pharmacologically assessed mAChR-A to monitor intracellular calcium mobilization upon receptor activation. In vivo injection of vesamicol-a VAChT blocker-at the cholinergic synapse, suppressed forced water uptake by desiccated ticks, while injection of atropine, an mAChR-A antagonist, did not show any effect on water volume uptake. This study has uncovered a novel neurotransmitter signaling pathway in Ixodes SG, and suggests its role in water uptake by type I acini in desiccated ticks.
Identifiants
pubmed: 32994503
doi: 10.1038/s41598-020-73077-1
pii: 10.1038/s41598-020-73077-1
pmc: PMC7524744
doi:
Substances chimiques
Cholinergic Agents
0
RNA, Messenger
0
Vesicular Acetylcholine Transport Proteins
0
Choline O-Acetyltransferase
EC 2.3.1.6
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
16054Références
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