Modern trends in downstream processing of biotherapeutics through continuous chromatography: The potential of Multicolumn Countercurrent Solvent Gradient Purification.
Biopharmaceuticals
Biotherapeutics
Continuous chromatography
Multicolumn platforms
Preparative chromatography
Purification
Journal
Trends in analytical chemistry : TRAC
ISSN: 0165-9936
Titre abrégé: Trends Analyt Chem
Pays: Netherlands
ID NLM: 8105858
Informations de publication
Date de publication:
Nov 2020
Nov 2020
Historique:
pubmed:
1
10
2020
medline:
1
10
2020
entrez:
30
9
2020
Statut:
ppublish
Résumé
Single-column (batch) preparative chromatography is the technique of choice for purification of biotherapeutics but it is often characterized by an intrinsic limitation in terms of yield-purity trade-off, especially for separations containing a larger number of product-related impurities. This drawback can be alleviated by employing multicolumn continuous chromatography. Among the different methods working in continuous mode, in this paper we will focus in particular on Multicolumn Countercurrent Solvent Gradient Purification (MCSGP) which has been specifically designed for challenging separations of target biomolecules from their product-related impurities. The improvements come from the automatic internal recycling of the impure fractions inside the chromatographic system, which results in an increased yield without compromising the purity of the pool. In this article, steps of the manufacturing process of biopharmaceuticals will be described, as well as the advantages of continuous chromatography over batch processes, by particularly focusing on MCSGP.
Identifiants
pubmed: 32994652
doi: 10.1016/j.trac.2020.116051
pii: S0165-9936(20)30280-6
pmc: PMC7513800
doi:
Types de publication
Journal Article
Review
Langues
eng
Pagination
116051Informations de copyright
© 2020 Published by Elsevier B.V.
Déclaration de conflit d'intérêts
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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