A 2-kb Mycovirus Converts a Pathogenic Fungus into a Beneficial Endophyte for Brassica Protection and Yield Enhancement.

Ascomycota / pathogenicity Brassica / microbiology Brassica napus / microbiology Circadian Rhythm / physiology Endophytes / physiology Flowers / microbiology Fungal Viruses / physiology
Brassica napus Sclerotinia sclerotiorum biological control endophyte mycovirus rapeseed stem rot

Journal

Molecular plant
ISSN: 1752-9867
Titre abrégé: Mol Plant
Pays: England
ID NLM: 101465514

Informations de publication

Date de publication:
05 10 2020
Historique:
received: 04 08 2020
revised: 28 08 2020
accepted: 29 08 2020
pubmed: 1 10 2020
medline: 22 6 2021
entrez: 30 9 2020
Statut: ppublish

Résumé

Mycoviruses are viruses that infect fungi, and hypovirulence-associated mycoviruses have the potential to control fungal diseases. However, it is unclear how mycovirus-mediated hypovirulent strains live and survive in the field, and no mycovirus has been applied for field crop protection. In this study, we found that a previously identified small DNA mycovirus (SsHADV-1) can convert its host, Sclerotinia sclerotiorum, from a typical necrotrophic pathogen to a beneficial endophytic fungus. SsHADV-1 downregulates the expression of key pathogenicity factor genes in S. sclerotiorum during infection. When growing in rapeseed, the SsHADV-1-infected strain DT-8 significantly regulates the expression of rapeseed genes involved in defense, hormone signaling, and circadian rhythm pathways. As a result, plant growth is promoted and disease resistance is enhanced. Field experiments showed that spraying DT-8 at the early flowering stage can reduce the disease severity of rapeseed stem rot by 67.6% and improve yield by 14.9%. Moreover, we discovered that SsHADV-1 could also infect other S. sclerotiorum strains on DT-8-inoculated plants and that DT-8 could be recovered from dead plants. These findings suggest that the mycoviruses may have the ability to shape the origin of endophytism. Our discoveries suggest that mycoviruses may influence the origin of endophytism and may also offer a novel strategy for disease control in which mycovirus-infected strains are used to improve crop health and release mycoviruses into the field.

Identifiants

DOI: 10.1016/j.molp.2020.08.016 PMID: 32998002
pubmed: 32998002
pii: S1674-2052(20)30293-8
doi: 10.1016/j.molp.2020.08.016
pii:
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1420-1433

Commentaires et corrections

Type : CommentIn

Informations de copyright

Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.

Auteurs

Hongxiang Zhang (H)

State Key Laboratory of Agricultural Microbiology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei Province 430070, China; The Provincial Key Lab of Plant Pathology of Hubei Province, Huazhong Agricultural University, Wuhan, China.

Jiatao Xie (J)

State Key Laboratory of Agricultural Microbiology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei Province 430070, China; The Provincial Key Lab of Plant Pathology of Hubei Province, Huazhong Agricultural University, Wuhan, China.

Yanping Fu (Y)

The Provincial Key Lab of Plant Pathology of Hubei Province, Huazhong Agricultural University, Wuhan, China.

Jiasen Cheng (J)

State Key Laboratory of Agricultural Microbiology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei Province 430070, China; The Provincial Key Lab of Plant Pathology of Hubei Province, Huazhong Agricultural University, Wuhan, China.

Zheng Qu (Z)

State Key Laboratory of Agricultural Microbiology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei Province 430070, China; The Provincial Key Lab of Plant Pathology of Hubei Province, Huazhong Agricultural University, Wuhan, China.

Zhenzhen Zhao (Z)

State Key Laboratory of Agricultural Microbiology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei Province 430070, China; The Provincial Key Lab of Plant Pathology of Hubei Province, Huazhong Agricultural University, Wuhan, China.

Shufen Cheng (S)

State Key Laboratory of Agricultural Microbiology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei Province 430070, China; The Provincial Key Lab of Plant Pathology of Hubei Province, Huazhong Agricultural University, Wuhan, China.

Tao Chen (T)

The Provincial Key Lab of Plant Pathology of Hubei Province, Huazhong Agricultural University, Wuhan, China.

Bo Li (B)

State Key Laboratory of Agricultural Microbiology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei Province 430070, China; The Provincial Key Lab of Plant Pathology of Hubei Province, Huazhong Agricultural University, Wuhan, China.

Qianqian Wang (Q)

State Key Laboratory of Agricultural Microbiology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei Province 430070, China; The Provincial Key Lab of Plant Pathology of Hubei Province, Huazhong Agricultural University, Wuhan, China.

Xinqiang Liu (X)

State Key Laboratory of Agricultural Microbiology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei Province 430070, China; The Provincial Key Lab of Plant Pathology of Hubei Province, Huazhong Agricultural University, Wuhan, China.

Binnian Tian (B)

State Key Laboratory of Agricultural Microbiology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei Province 430070, China; The Provincial Key Lab of Plant Pathology of Hubei Province, Huazhong Agricultural University, Wuhan, China.

David B Collinge (DB)

Department of Plant and Environmental Sciences, University of Copenhagen, 1871 Frederiksberg C, Denmark.

Daohong Jiang (D)

State Key Laboratory of Agricultural Microbiology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei Province 430070, China; The Provincial Key Lab of Plant Pathology of Hubei Province, Huazhong Agricultural University, Wuhan, China. Electronic address: daohongjiang@mail.hzau.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Champignons › Ascomycota › A 2-kb Mycovirus Converts a Pathogenic Fungus...
questionsmedicales.fr › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Magnoliopsida › Brassicaceae › Brassica › A 2-kb Mycovirus Converts a Pathogenic Fungus...
questionsmedicales.fr › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Magnoliopsida › Brassicaceae › Brassica › Brassica napus › A 2-kb Mycovirus Converts a Pathogenic Fungus...
questionsmedicales.fr › Phénomènes physiologiques › Phénomènes chronobiologiques › Périodicité › Rythme circadien › A 2-kb Mycovirus Converts a Pathogenic Fungus...
questionsmedicales.fr › Stades d'organismes › Endophytes › A 2-kb Mycovirus Converts a Pathogenic Fungus...
questionsmedicales.fr › Structures de plante › Parties aériennes de plante › Sommités fleuries › Fleurs › A 2-kb Mycovirus Converts a Pathogenic Fungus...
questionsmedicales.fr › Virus › Virus fongiques › A 2-kb Mycovirus Converts a Pathogenic Fungus...