Working with Bacterial Artificial Chromosomes (BACs) and Other High-Capacity Vectors.
Animals
Bacteriophage P1
/ genetics
Chromosomes, Artificial, Bacterial
/ genetics
Chromosomes, Artificial, Yeast
/ genetics
Escherichia coli
/ genetics
Gene Transfer Techniques
Genes, Reporter
/ genetics
Genetic Vectors
/ genetics
Green Fluorescent Proteins
/ genetics
Humans
Mice, Transgenic
Models, Genetic
Recombination, Genetic
/ genetics
Transgenes
/ genetics
Journal
Cold Spring Harbor protocols
ISSN: 1559-6095
Titre abrégé: Cold Spring Harb Protoc
Pays: United States
ID NLM: 101524530
Informations de publication
Date de publication:
01 10 2020
01 10 2020
Historique:
entrez:
2
10
2020
pubmed:
3
10
2020
medline:
5
10
2021
Statut:
epublish
Résumé
Genetic targeting of specific cell types is fundamentally important for modern molecular-genetic studies. The development of simple methods to engineer high-capacity vectors-in particular, bacterial artificial chromosomes (BACs)-for the preparation of transgenic lines that accurately express a gene of interest has resulted in commonplace usage of transgenic techniques in a wide variety of experimental systems. Here we provide a brief description of each of the four major types of large-capacity vectors, with a focus on the use of BAC vectors.
Identifiants
pubmed: 33004554
pii: 2020/10/pdb.top097998
doi: 10.1101/pdb.top097998
doi:
Substances chimiques
Green Fluorescent Proteins
147336-22-9
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2020 Cold Spring Harbor Laboratory Press.