Full-length TDP-43 and its C-terminal domain form filaments


Journal

Amyloid : the international journal of experimental and clinical investigation : the official journal of the International Society of Amyloidosis
ISSN: 1744-2818
Titre abrégé: Amyloid
Pays: England
ID NLM: 9433802

Informations de publication

Date de publication:
Mar 2021
Historique:
pubmed: 8 10 2020
medline: 16 11 2021
entrez: 7 10 2020
Statut: ppublish

Résumé

Accumulation of ubiquitin-positive, tau- and α-synuclein-negative intracellular inclusions of TDP-43 in the central nervous system represents the major hallmark correlated to amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration with ubiquitin-positive inclusions (FTLD-U). Such inclusions have variably been described as amorphous aggregates or more structured deposits having amyloid properties. Here we have purified full-length TDP-43 (FL TDP-43) and its C-terminal domain (Ct TDP-43) to investigate the morphological, structural and tinctorial features of aggregates formed

Identifiants

pubmed: 33026249
doi: 10.1080/13506129.2020.1826425
pmc: PMC7613275
mid: EMS151990
doi:

Substances chimiques

Amyloid 0
Amyloidogenic Proteins 0
DNA-Binding Proteins 0
TARDBP protein, human 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

56-65

Subventions

Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 206409
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 206409/Z/17/Z
Pays : United Kingdom
Organisme : Motor Neurone Disease Association
ID : CHRISTODOULOU/OCT15/882-792
Pays : United Kingdom

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Auteurs

Claudia Capitini (C)

Section of Biochemistry, Department of Experimental and Clinical Biomedical Sciences, University of Florence, Florence, Italy.
European Laboratory for Non-linear Spectroscopy, Sesto Fiorentino, Italy.

Giulia Fani (G)

Section of Biochemistry, Department of Experimental and Clinical Biomedical Sciences, University of Florence, Florence, Italy.

Mirella Vivoli Vega (M)

Section of Biochemistry, Department of Experimental and Clinical Biomedical Sciences, University of Florence, Florence, Italy.

Amanda Penco (A)

Department of Physics, University of Genoa, Genoa, Italy.

Claudio Canale (C)

Department of Physics, University of Genoa, Genoa, Italy.

Lisa D Cabrita (LD)

Institute of Structural and Molecular Biology, UCL and Birkbeck College London, London, UK.

Martino Calamai (M)

European Laboratory for Non-linear Spectroscopy, Sesto Fiorentino, Italy.
National Institute of Optics, National Research Council, Sesto Fiorentino, Italy.

John Christodoulou (J)

Institute of Structural and Molecular Biology, UCL and Birkbeck College London, London, UK.

Annalisa Relini (A)

Department of Physics, University of Genoa, Genoa, Italy.

Fabrizio Chiti (F)

Section of Biochemistry, Department of Experimental and Clinical Biomedical Sciences, University of Florence, Florence, Italy.

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Classifications MeSH