Dynamics of genomic and immune responses during primary immunotherapy resistance in mismatch repair-deficient tumors.


Journal

Cold Spring Harbor molecular case studies
ISSN: 2373-2873
Titre abrégé: Cold Spring Harb Mol Case Stud
Pays: United States
ID NLM: 101660017

Informations de publication

Date de publication:
10 2020
Historique:
received: 11 06 2020
accepted: 10 08 2020
entrez: 8 10 2020
pubmed: 9 10 2020
medline: 5 6 2021
Statut: epublish

Résumé

Mismatch repair-deficient (dMMR) cancers generate a substantial number of immunogenic neoantigens, rendering them sensitive to immunotherapy. Yet, there is considerable variability in responses, and roughly one-half of dMMR cancers are refractory to immunotherapy. Here we study a patient with dMMR lung cancer refractory to immunotherapy. The tumor exhibited typical dMMR molecular features, including exceptionally high frameshift insertions and deletions (indels). Despite the treatment inducing abundant intratumoral T-cell infiltrates, it failed to elicit tumor regression, pointing to the T cells lacking cytotoxic activity. A post-treatment tumor demonstrated compound heterozygous frameshift deletions located upstream of the kinase domain in the gene encoding JAK1 protein, down-regulation of JAK1 and mediators of its signal transduction, and total loss of JAK1 phosphorylation. Importantly, one of the

Identifiants

pubmed: 33028646
pii: mcs.a005678
doi: 10.1101/mcs.a005678
pmc: PMC7552928
pii:
doi:

Substances chimiques

Biomarkers, Tumor 0
MLH1 protein, human 0
JAK1 protein, human EC 2.7.10.2
Janus Kinase 1 EC 2.7.10.2
MutL Protein Homolog 1 EC 3.6.1.3

Types de publication

Case Reports Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

© 2020 Takahashi et al.; Published by Cold Spring Harbor Laboratory Press.

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Auteurs

Nobuyuki Takahashi (N)

Developmental Therapeutics Branch, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892, USA.

Vinodh N Rajapakse (VN)

Developmental Therapeutics Branch, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892, USA.

Lorinc Pongor (L)

Developmental Therapeutics Branch, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892, USA.

Suresh Kumar (S)

Developmental Therapeutics Branch, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892, USA.

Camille Tlemsani (C)

Developmental Therapeutics Branch, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892, USA.

Rebecca Erwin-Cohen (R)

Cancer and Inflammation Program, Center for Cancer Research, National Cancer Institute, Frederick, Maryland 21702, USA.

Howard A Young (HA)

Laboratory of Cancer Immunometabolism, Center for Cancer Research, National Cancer Institute, Frederick, Maryland 21702, USA.

Stephen Hewitt (S)

Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892, USA.

Jun S Wei (JS)

Genetics Branch, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892, USA.

Javed Khan (J)

Genetics Branch, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892, USA.

Alejandro V Villarino (AV)

Department of Microbiology and Immunology, Miller School of Medicine, University of Miami, Miami, Florida 33136, USA.

Jane B Trepel (JB)

Developmental Therapeutics Branch, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892, USA.

Anish Thomas (A)

Developmental Therapeutics Branch, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland 20892, USA.

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Classifications MeSH