Cisplatin-Induced Stria Vascularis Damage Is Associated with Inflammation and Fibrosis.
Journal
Neural plasticity
ISSN: 1687-5443
Titre abrégé: Neural Plast
Pays: United States
ID NLM: 100883417
Informations de publication
Date de publication:
2020
2020
Historique:
received:
19
04
2020
revised:
05
08
2020
accepted:
03
09
2020
entrez:
8
10
2020
pubmed:
9
10
2020
medline:
10
9
2021
Statut:
epublish
Résumé
The stria vascularis (SV) generates the endocochlear potential (EP) in the inner ear and is necessary for proper hair cell (HC) mechanotransduction and hearing. Cell junctions are indispensable for the establishment of compositionally distinct fluid compartments in the inner ear. Ototoxic drug cisplatin can damage SV and cause sensorineural hearing loss; however, the underlying mechanisms behind such injury are unclear. In this study, after the intraperitoneal injection of cisplatin (3 mg/kg/day for 7 days) in mice, we determined the auditory function by EP recording and auditory brainstem response (ABR) analysis, observed the ultrastructure of SV by transmission electron microscopy (TEM), and examined the expression and distribution of cell junction proteins by western blot, PCR, and immunofluorescence staining. We discovered that the EP was significantly reduced while ABR thresholds were significantly elevated in cisplatin-treated mice; cisplatin induced ultrastructural changes in marginal cells (MCs), endothelial cells (ECs), pericytes, etc. We found that cisplatin insulted auditory function not only by reducing the expression of zonula occludens protein-1 (ZO-1) in MCs of the SV but also by decreasing the expression of connexin 26 (Cx26) and connexin 43 (Cx43) in MCs and basal cells (BCs). More importantly, cisplatin induced activations of perivascular-resident macrophage-like melanocytes (PVM/Ms) and interleukin-1beta (IL-1
Identifiants
pubmed: 33029120
doi: 10.1155/2020/8851525
pmc: PMC7527906
doi:
Substances chimiques
Cisplatin
Q20Q21Q62J
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
8851525Informations de copyright
Copyright © 2020 Na Zhang et al.
Déclaration de conflit d'intérêts
The authors declare that there are no conflicts of interest regarding the publication of this paper.
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