In vivo CRISPR/Cas9 knockout screen: TCEAL1 silencing enhances docetaxel efficacy in prostate cancer.
Animals
CRISPR-Cas Systems
/ genetics
Cell Line, Tumor
Clustered Regularly Interspaced Short Palindromic Repeats
/ genetics
DNA-Binding Proteins
/ genetics
Docetaxel
/ pharmacology
Drug Resistance, Neoplasm
/ genetics
Gene Expression Regulation, Neoplastic
/ genetics
Genetic Engineering
/ methods
Male
Mice
Mice, Nude
Prostatic Neoplasms
/ genetics
Taxoids
/ pharmacology
Transcription Factors
/ genetics
Xenograft Model Antitumor Assays
/ methods
Journal
Life science alliance
ISSN: 2575-1077
Titre abrégé: Life Sci Alliance
Pays: United States
ID NLM: 101728869
Informations de publication
Date de publication:
12 2020
12 2020
Historique:
received:
08
05
2020
revised:
22
09
2020
accepted:
25
09
2020
entrez:
9
10
2020
pubmed:
10
10
2020
medline:
14
7
2021
Statut:
epublish
Résumé
Docetaxel chemotherapy in metastatic prostate cancer offers only a modest survival benefit because of emerging resistance. To identify candidate therapeutic gene targets, we applied a murine prostate cancer orthograft model that recapitulates clinical invasive prostate cancer in a genome-wide CRISPR/Cas9 screen under docetaxel treatment pressure. We identified 17 candidate genes whose suppression may enhance the efficacy of docetaxel, with transcription elongation factor A-like 1 (
Identifiants
pubmed: 33033111
pii: 3/12/e202000770
doi: 10.26508/lsa.202000770
pmc: PMC7556750
pii:
doi:
Substances chimiques
DNA-Binding Proteins
0
TCEAL1 protein, human
0
Taxoids
0
Transcription Factors
0
Docetaxel
15H5577CQD
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2020 Rushworth et al.
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