The specific metabolome profiling of patients infected by SARS-COV-2 supports the key role of tryptophan-nicotinamide pathway and cytosine metabolism.

Aged Aged, 80 and over Betacoronavirus / genetics Biomarkers / blood COVID-19 Coronavirus Infections / diagnosis Cytosine / blood Early Diagnosis Female France / epidemiology Humans Male Metabolome Metabolomics / methods Middle Aged Niacinamide / blood Pandemics Pneumonia, Viral / diagnosis Prognosis Reproducibility of Results SARS-CoV-2 Sensitivity and Specificity Severity of Illness Index Tryptophan / blood

Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
08 10 2020
Historique:
received: 13 07 2020
accepted: 21 09 2020
entrez: 9 10 2020
pubmed: 10 10 2020
medline: 28 10 2020
Statut: epublish

Résumé

The biological mechanisms involved in SARS-CoV-2 infection are only partially understood. Thus we explored the plasma metabolome of patients infected with SARS-CoV-2 to search for diagnostic and/or prognostic biomarkers and to improve the knowledge of metabolic disturbance in this infection. We analyzed the plasma metabolome of 55 patients infected with SARS-CoV-2 and 45 controls by LC-HRMS at the time of viral diagnosis (D0). We first evaluated the ability to predict the diagnosis from the metabotype at D0 in an independent population. Next, we assessed the feasibility of predicting the disease evolution at the 7th and 15th day. Plasma metabolome allowed us to generate a discriminant multivariate model to predict the diagnosis of SARS-CoV-2 in an independent population (accuracy > 74%, sensitivity, specificity > 75%). We identified the role of the cytosine and tryptophan-nicotinamide pathways in this discrimination. However, metabolomic exploration modestly explained the disease evolution. Here, we present the first metabolomic study in SARS-CoV-2 patients which showed a high reliable prediction of early diagnosis. We have highlighted the role of the tryptophan-nicotinamide pathway clearly linked to inflammatory signals and microbiota, and the involvement of cytosine, previously described as a coordinator of cell metabolism in SARS-CoV-2. These findings could open new therapeutic perspectives as indirect targets.

Identifiants

DOI: 10.1038/s41598-020-73966-5 PMID: 33033346 PMC: PMC7544910
pubmed: 33033346
doi: 10.1038/s41598-020-73966-5
pii: 10.1038/s41598-020-73966-5
pmc: PMC7544910
doi:

Substances chimiques

Biomarkers 0
Niacinamide 25X51I8RD4
Tryptophan 8DUH1N11BX
Cytosine 8J337D1HZY

Types de publication

Evaluation Study Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

16824

Références

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Auteurs

H Blasco (H)

UMR 1253, iBrain, équipe « neurogénomique et physiopathologie neuronale », INSERM, Université de Tours, Tours, France. helene.blasco@univ-tours.fr.
Service de Biochimie et Biologie Moléculaire, CHU de Tours, Tours, France. helene.blasco@univ-tours.fr.

C Bessy (C)

Service de Pneumologie et d'Explorations Fonctionnelles Respiratoires, CHU, Tours, France.

L Plantier (L)

Service de Pneumologie et d'Explorations Fonctionnelles Respiratoires, CHU, Tours, France.
Centre d'Etude Des Pathologies Respiratoires, INSERM U1100, Université de Tours, Tours, France.

A Lefevre (A)

UMR 1253, iBrain, équipe « neurogénomique et physiopathologie neuronale », INSERM, Université de Tours, Tours, France.

E Piver (E)

Service de Biochimie et Biologie Moléculaire, CHU de Tours, Tours, France.
INSERM U1259, MAVIVH, Université de Tours, Tours, France.

L Bernard (L)

Service de Médecine Interne et Maladies Infectieuses, CHU de Tours, Tours, France.

J Marlet (J)

INSERM U1259, MAVIVH, Université de Tours, Tours, France.
Service de Bactériologie-Virologie-Hygiène, CHU de Tours, Tours, France.

K Stefic (K)

INSERM U1259, MAVIVH, Université de Tours, Tours, France.
Service de Bactériologie-Virologie-Hygiène, CHU de Tours, Tours, France.

Isabelle Benz-de Bretagne (I)

UMR 1253, iBrain, équipe « neurogénomique et physiopathologie neuronale », INSERM, Université de Tours, Tours, France.
Service de Biochimie et Biologie Moléculaire, CHU de Tours, Tours, France.

P Cannet (P)

Service de Biochimie et Biologie Moléculaire, CHU de Tours, Tours, France.

H Lumbu (H)

Service de Biochimie et Biologie Moléculaire, CHU de Tours, Tours, France.

T Morel (T)

Service de Biochimie et Biologie Moléculaire, CHU de Tours, Tours, France.

P Boulard (P)

Service de Biochimie et Biologie Moléculaire, CHU de Tours, Tours, France.

C R Andres (CR)

UMR 1253, iBrain, équipe « neurogénomique et physiopathologie neuronale », INSERM, Université de Tours, Tours, France.
Service de Biochimie et Biologie Moléculaire, CHU de Tours, Tours, France.

P Vourc'h (P)

UMR 1253, iBrain, équipe « neurogénomique et physiopathologie neuronale », INSERM, Université de Tours, Tours, France.
Service de Biochimie et Biologie Moléculaire, CHU de Tours, Tours, France.

O Hérault (O)

Department of Biological Hematology, University Hospital of Tours, Tours, France.
CNRS ERL 7001 LNOx and EA, University of Tours, 7501, Tours, France.

A Guillon (A)

Centre d'Etude Des Pathologies Respiratoires, INSERM U1100, Université de Tours, Tours, France.
Intensive Care Unit, Research Center for Respiratory, Tours University Hospital, Tours, France.

P Emond (P)

UMR 1253, iBrain, équipe « neurogénomique et physiopathologie neuronale », INSERM, Université de Tours, Tours, France.
Service de Médecine Nucléaire in vitro, CHRU de Tours, Tours, France.

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Classifications MeSH

questionsmedicales.fr › Personnes › Tranches d'âge › Adulte › Sujet âgé › The specific metabolome profiling of patients...
questionsmedicales.fr › Personnes › Tranches d'âge › Adulte › Sujet âgé › Sujet âgé de 80 ans ou plus › The specific metabolome profiling of patients...
questionsmedicales.fr › Virus › Virus à ARN › Virus à ARN à brin positif › Nidovirales › Coronaviridae › Coronavirus › Betacoronavirus › The specific metabolome profiling of patients...
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questionsmedicales.fr › Maladies de l'appareil respiratoire › Infections de l'appareil respiratoire › Pneumopathie infectieuse › Pneumopathie virale › COVID-19 › The specific metabolome profiling of patients...
questionsmedicales.fr › Infection › Maladies virales › Infections à virus à ARN › Infections à Nidovirales › Infections à Coronaviridae › Infections à coronavirus › The specific metabolome profiling of patients...
questionsmedicales.fr › Composés hétérocycliques › Composés hétéromonocycliques › Pyrimidines › Pyrimidinones › Cytosine › The specific metabolome profiling of patients...
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questionsmedicales.fr › Composés hétérocycliques › Composés hétéromonocycliques › Pyridines › Acides nicotiniques › Nicotinamide › The specific metabolome profiling of patients...
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questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Méthodologie en recherche épidémiologique › Reproductibilité des résultats › The specific metabolome profiling of patients...
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