Transcriptional programs of infectious spleen and kidney necrosis virus (ISKNV) in vitro and in vivo.
Expression profile
Infectious spleen and kidney necrosis virus
Iridovirus
Megalocytivirus
Transcriptional program
Journal
Virus genes
ISSN: 1572-994X
Titre abrégé: Virus Genes
Pays: United States
ID NLM: 8803967
Informations de publication
Date de publication:
Dec 2020
Dec 2020
Historique:
received:
14
05
2020
accepted:
26
09
2020
pubmed:
10
10
2020
medline:
22
7
2021
entrez:
9
10
2020
Statut:
ppublish
Résumé
Infectious spleen and kidney necrosis virus (ISKNV), causing serious infectious diseases to marine and freshwater fishes, is the type species of the genus Megalocytivirus, family Iridoviridae. In this study, the transcriptional programs of ISKNV in vitro (MFF-1 cells) and in vivo (spleens from mandarin fish) were investigated using real-time PCR. Transcription of all the putative open reading frames (ORFs) of ISKNV was verified. The temporal expression patterns of ISKNV ORFs in vitro and in vivo, including peak expression times (PETs) and relative maximal expression levels, were determined and compared. The K-means clustering with Spearman rank correlation was generated in heat maps constructed based on ISKNV ORF expression profiles in vivo and in vitro. The current study may provide a global picture of ISKNV infection at the transcription level and help better understand the molecular pathogenic mechanism of megalocytiviruses.
Identifiants
pubmed: 33033883
doi: 10.1007/s11262-020-01800-1
pii: 10.1007/s11262-020-01800-1
doi:
Substances chimiques
Viral Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
749-755Subventions
Organisme : National Key Research and Development Program of China
ID : 2018YFD0900505
Références
Darai G, Anders K, Koch HG, Delius H, Gelderblom H, Samalecos C, Flugel RM (1983) Analysis of the genome of fish lymphocystis disease virus isolated directly from epidermal tumours of pleuronectes. Virology 126:466–479
doi: 10.1016/S0042-6822(83)80005-1
Williams T (2008) Natural invertebrate hosts of iridoviruses (Iridoviridae). Neotrop Entomol 37:615–632
doi: 10.1590/S1519-566X2008000600001
Zhang QY, Gui JF (2015) Virus genomes and virus-host interactions in aquaculture animals. Sci China Life Sci 58:156–169
doi: 10.1007/s11427-015-4802-y
King AMQ (2012) classification and nomenclature of viruses. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (eds) Virus taxonomy of the International Committee on Taxonomy of Viruses. Elsevier Academic Press, London, pp 806–828
Kurita J, Nakajima K (2012) Megalocytiviruses Viruses 4:521–538
doi: 10.3390/v4040521
Wang YQ, Lu L, Weng SP, Huang JN, Chan SM, He JG (2007) Molecular epidemiology and phylogenetic analysis of a marine fish infectious spleen and kidney necrosis virus-like (ISKNV-like) virus. Arch Virol 152:763–773
doi: 10.1007/s00705-006-0870-4
Jeong JB, Kim HY, Jun LJ, Lyu JH, Park NG, Kim JK, Jeong HD (2008) Outbreaks and risks of infectious spleen and kidney necrosis virus disease in freshwater ornamental fishes. Dis Aquat Organ 78:209–215
doi: 10.3354/dao01879
Johnson SJ, Hick PM, Robinson AP, Rimmer AE, Tweedie A, Becker JA (2019) The impact of pooling samples on surveillance sensitivity for the Megalocytivirus infectious spleen and kidney necrosis virus. Transbound Emerg Dis 66:2318–2328
doi: 10.1111/tbed.13288
Xu X, Huang L, Weng S, Wang J, Lin T, Tang J, Li Z, Lu Q, Xia Q, Yu X, He J (2010) Tetraodon nigroviridis as a nonlethal model of infectious spleen and kidney necrosis virus (ISKNV) infection. Virology 406:167–175
doi: 10.1016/j.virol.2010.07.003
Xu X, Zhang L, Weng S, Huang Z, Lu J, Lan D, Zhong X, Yu X, Xu A, He J (2008) A zebrafish (Danio rerio) model of infectious spleen and kidney necrosis virus (ISKNV) infection. Virology 376:1–12
doi: 10.1016/j.virol.2007.12.026
He JG, Deng M, Weng SP, Li Z, Zhou SY, Long QX, Wang XZ, Chan SM (2001) Complete genome analysis of the mandarin fish infectious spleen and kidney necrosis Iridovirus. Virology 291:126–139
doi: 10.1006/viro.2001.1208
Ince IA, Boeren S, van Oers MM, Vlak JM (2015) Temporal proteomic analysis and label-free quantification of viral proteins of an invertebrate Iridovirus. J Gen Virol 96:196–205
doi: 10.1099/vir.0.068312-0
Ke F, Gui JF, Chen ZY, Li T, Lei CK, Wang ZH, Zhang QY (2018) Divergent transcriptomic responses underlying the ranaviruses-amphibian interaction processes on interspecies infection of Chinese giant salamander. BMC Genomics 19:211
doi: 10.1186/s12864-018-4596-y
Ince IA, Pijlman GP, Vlak JM, van Oers MM (2017) Hairpin structures with conserved sequence motifs determine the 3' ends of non-polyadenylated invertebrate iridovirus transcripts. Virology 511:344–353
doi: 10.1016/j.virol.2017.06.026
Wu Q, Ning X, Jiang S, Sun L (2020) Transcriptome analysis reveals seven key immune pathways of Japanese flounder (Paralichthys olivaceus) involved in Megalocytivirus infection. Fish Shellfish Immunol 103:150–158
doi: 10.1016/j.fsi.2020.05.011
Zhang J, Sun L (2017) Transcriptome analysis reveals temperature-regulated antiviral response in turbot Scophthalmus maximus. Fish Shellfish Immunol 68:359–367
doi: 10.1016/j.fsi.2017.07.038
Chinchar VG, Hyatt A, Miyazaki T, Williams T (2009) Family iridoviridae: poor viral relations no longer. Curr Top Microbiol Immunol 328:123–170
pubmed: 19216437
Lieberman PM (2008) Chromatin organization and virus gene expression. J Cell Physiol 216:295–302
doi: 10.1002/jcp.21421
Ho WJ, Danilova L, Lim SJ, Verma R, Xavier S, Leatherman JM, Sztein MB, Fertig EJ, Wang H, Jaffee E, Yarchoan M (2020) Viral status, immune microenvironment and immunological response to checkpoint inhibitors in hepatocellular carcinoma. J Immunother Cancer 8(1):e000394
doi: 10.1136/jitc-2019-000394
Dong C, Weng S, Shi X, Xu X, Shi N, He J (2008) Development of a mandarin fish Siniperca chuatsi fry cell line suitable for the study of infectious spleen and kidney necrosis virus (ISKNV). Virus Res 135:273–281
doi: 10.1016/j.virusres.2008.04.004
Li Z, Xu X, Huang L, Wu J, Lu Q, Xiang Z, Liao J, Weng S, Yu X, He J (2010) Administration of recombinant IFN1 protects zebrafish (Danio rerio) from ISKNV infection. Fish Shellfish Immunol 29:399–406
doi: 10.1016/j.fsi.2010.04.020
Dong CF, Xiong XP, Shuang F, Weng SP, Zhang J, Zhang Y, Luo YW, He JG (2011) Global landscape of structural proteins of infectious spleen and kidney necrosis virus. J Virol 85:869–2877
doi: 10.1128/JVI.01444-10
Chen LM, Wang F, Song W, Hew CL (2006) Temporal and differential gene expression of Singapore grouper iridovirus. J Gen Virol 87:2907–2915
doi: 10.1099/vir.0.82219-0
Teng Y, Hou Z, Gong J, Liu H, Xie X, Zhang L, Chen X, Qin QW (2008) Whole-genome transcriptional profiles of a novel marine fish iridovirus, Singapore grouper iridovirus (SGIV) in virus-infected grouper spleen cell cultures and in orange-spotted grouper, Epinephulus coioides. Virology 377:39–48
doi: 10.1016/j.virol.2008.04.011
D'Costa SM, Yao H, Bilimoria SL (2001) Transcription and temporal cascade in Chiloiridescent virus infected cells. Arch Virol 146:2165–2178
doi: 10.1007/s007050170027
Lua DT, Yasuike M, Hirono I, Aoki T (2005) Transcription program of red sea bream iridovirus as revealed by DNA microarrays. J Virol 79:15151–15164
doi: 10.1128/JVI.79.24.15151-15164.2005
Paulose-Murphy M, Ha NK, Xiang C, Chen Y, Gillim L, Yarchoan R, Meltzer P, Bittner M, Trent J, Zeichner S (2001) Transcription program of human herpesvirus 8 (Kaposi's Sarcoma-Associated Herpesvirus). J Virol 75:4843–4853
doi: 10.1128/JVI.75.10.4843-4853.2001
Ahn JW, Powell KL, Kellam P, Alber DG (2002) Gammaherpesvirus lytic gene expression as characterized by DNA array. J Virol 76:6244–6256
doi: 10.1128/JVI.76.12.6244-6256.2002
Tsai JM, Wang HC, Leu JH, Hsiao HH, Wang AH, Kou GH, Lo CF (2004) Genomic and proteomic analysis of thirty-nine structural proteins of shrimp white spot syndrome virus. J Virol 78:11360–11370
doi: 10.1128/JVI.78.20.11360-11370.2004
Yoo SM, Zhou FC, Ye FC, Pan HY, Gao SJ (2005) Early and sustained expression of latent and host modulating genes in coordinated transcriptional program of KSHV productive primary infection of human primary endothelial cells. Virology 343:47–64
doi: 10.1016/j.virol.2005.08.018
Song JJ, Lee HJ, Morris JS, Kang S (2007) Clustering of time-course gene expression data using functional data analysis. Comput Biol Chem 31:265–274
doi: 10.1016/j.compbiolchem.2007.05.006
Majji S, Thodima V, Sample R, Whitley D, Deng Y, Mao J, Chinchar VG (2009) Transcriptome analysis of Frog virus 3, the type species of the genus Ranavirus, family Iridoviridae. Virology 391:293–303
doi: 10.1016/j.virol.2009.06.022
Oshima S, Hata J, Hirasawa N, Ohtaka T, Hirono I, Aoki T, Yamashita S (1998) Rapid diagnosis of red sea bream iridovirus infection using the polymerase chain reaction. Dis Aquat Organ 32:87–90
doi: 10.3354/dao032087
Dang TL, Yasuike M, Hirono I, Kondo H, Aoki T (2007) Transcriptional profile of red seabream iridovirus in a fish model as revealed by viral DNA microarrays. Virus Genes 35:449–461
doi: 10.1007/s11262-007-0090-3
Xu X, Weng S, Lin T, Tang J, Huang L, Wang J, Yu X, Lu L, Huang Z, He J (2010) VP23R of infectious spleen and kidney necrosis virus mediates formation of virus-mock basement membrane to provide attaching sites for lymphatic endothelial cells. J Virol 84:11866–11875
doi: 10.1128/JVI.00990-10
Wang ZL, Xu XP, He BL, Weng SP, Xiao J, Wang L, Lin T, Liu X, Wang Q, Yu XQ, He JG (2008) Infectious spleen and kidney necrosis virus orf48r functions as a new viral vascular endothelial growth factor. J Virol 82:4371–4383
doi: 10.1128/JVI.02027-07
Yuan JM, He BL, Yang LY, Guo CJ, Weng SP, Li SC, He JG (2015) Interaction of infectious spleen and kidney necrosis virus ORF119L with PINCH leads to dominant-negative inhibition of integrin-linked kinase and cardiovascular defects in zebrafish. J Virol 89:763–775
doi: 10.1128/JVI.01955-14
D'Haeseleer P (2005) How does gene expression clustering work? Nat Biotechnol 23:1499–1501
doi: 10.1038/nbt1205-1499
Papin J, Vahrson W, Hines-Boykin R, Dittmer DP (2005) Real-time quantitative PCR analysis of viral transcription. Methods Mol Biol 292:449–480
pubmed: 15507725