Transcription factor Kruppel-like factor 5 positively regulates the expression of AarF domain containing kinase 4.
A549 Cells
Child
Child, Preschool
Female
Gene Expression Regulation
HEK293 Cells
HeLa Cells
Humans
Kruppel-Like Transcription Factors
/ genetics
Male
Nephrotic Syndrome
/ genetics
Podocytes
/ metabolism
Promoter Regions, Genetic
/ genetics
Protein Kinases
/ genetics
RNA, Messenger
/ genetics
Transcription, Genetic
Up-Regulation
AarF domain containing kinase 4
Kruppel-like factor 5
Nephrotic syndrome
Promoter
Transcriptional regulation
Journal
Molecular biology reports
ISSN: 1573-4978
Titre abrégé: Mol Biol Rep
Pays: Netherlands
ID NLM: 0403234
Informations de publication
Date de publication:
Nov 2020
Nov 2020
Historique:
received:
27
05
2020
accepted:
30
09
2020
revised:
26
09
2020
pubmed:
10
10
2020
medline:
25
5
2021
entrez:
9
10
2020
Statut:
ppublish
Résumé
AarF domain containing kinase 4 (ADCK4) is identified as a candidate gene associated with hereditary nephrotic syndrome (NS). Kruppel-like factor 5 (KLF5) is reported to promote podocyte survival by blocking the ERK/p38 MAPK pathways. Both ADCK4 and KLF5 are involved in the occurrence and development of podocyte disease, but their interaction remains unclear. Firstly, we found that the mRNA levels of ADCK4 and KLF5 decreased in NS patients, and both levels showed an obvious linear relationship. Secondly, we cloned the ADCK4 promoter region and examined its promoter activity in Hela, A549, and HEK 293 cell lines. Deletion analysis showed that the region - 116/- 4 relative to the transcriptional start site (TSS) was the core region of ADCK4 promoter. Thirdly, mutation analysis showed that putative binding sites for KLF5 contributed to the ADCK4 promoter activity. In HEK293 cells, we found that KLF5 upregulated the mRNA and protein levels of ADCK4. Finally, our chromatin immunoprecipitation assay found that KLF5 could bind to the specific region of ADCK4 promoter. These results showed that KLF5 can positively regulate the transcriptional activity of ADCK4.
Identifiants
pubmed: 33033902
doi: 10.1007/s11033-020-05882-w
pii: 10.1007/s11033-020-05882-w
doi:
Substances chimiques
KLF5 protein, human
0
Kruppel-Like Transcription Factors
0
RNA, Messenger
0
COQ8B protein, human
EC 2.7.-
Protein Kinases
EC 2.7.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
8419-8427Subventions
Organisme : National Natural Science Foundation of China
ID : No. 81970579
Organisme : National Natural Science Foundation of China
ID : No. 81972954
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