Using Aptamers as a Novel Method for Determining GnRH/LH Pulsatility.
aptamer
gonadotropin-releasing hormone (GnRH), luteinizing hormone (LH), pulsatility
synthetic oligonucleotides
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
07 Oct 2020
07 Oct 2020
Historique:
received:
07
08
2020
revised:
29
09
2020
accepted:
05
10
2020
entrez:
10
10
2020
pubmed:
11
10
2020
medline:
2
3
2021
Statut:
epublish
Résumé
Aptamers are a novel technology enabling the continuous measurement of analytes in blood and other body compartments, without the need for repeated sampling and the associated reagent costs of traditional antibody-based methodologies. Aptamers are short single-stranded synthetic RNA or DNA that recognise and bind to specific targets. The conformational changes that can occur upon aptamer-ligand binding are transformed into chemical, fluorescent, colour changes and other readouts. Aptamers have been developed to detect and measure a variety of targets in vitro and in vivo. Gonadotropin-releasing hormone (GnRH) is a pulsatile hypothalamic hormone that is essential for normal fertility but difficult to measure in the peripheral circulation. However, pulsatile GnRH release results in pulsatile luteinizing hormone (LH) release from the pituitary gland. As such, LH pulsatility is the clinical gold standard method to determine GnRH pulsatility in humans. Aptamers have recently been shown to successfully bind to and measure GnRH and LH, and this review will focus on this specific area. However, due to the adaptability of aptamers, and their suitability for incorporation into portable devices, aptamer-based technology is likely to be used more widely in the future.
Identifiants
pubmed: 33036411
pii: ijms21197394
doi: 10.3390/ijms21197394
pmc: PMC7582658
pii:
doi:
Substances chimiques
Aptamers, Nucleotide
0
Biomarkers
0
Gonadotropin-Releasing Hormone
33515-09-2
Luteinizing Hormone
9002-67-9
Types de publication
Journal Article
Review
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Department of Health
ID : CS-2018-18-ST2-002
Pays : United Kingdom
Organisme : National Institute for Health Research
ID : RP-2014-05-001
Organisme : National Institute for Health Research
ID : National Institute for Health Research
Organisme : Imperial-BRC IPPRF
ID : P79696
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