Host Systems for the Production of Recombinant Spider Silk.
production systems
spider silk
spidroin
synthetic biology
Journal
Trends in biotechnology
ISSN: 1879-3096
Titre abrégé: Trends Biotechnol
Pays: England
ID NLM: 8310903
Informations de publication
Date de publication:
06 2021
06 2021
Historique:
received:
02
06
2020
revised:
16
09
2020
accepted:
17
09
2020
pubmed:
15
10
2020
medline:
7
10
2021
entrez:
14
10
2020
Statut:
ppublish
Résumé
Spider silk is renowned for its impressive mechanical properties. It is one of the strongest known biomaterials, possessing mechanical properties that outmatch both steel and Kevlar. However, the farming of spiders for their silk is unfeasible. Consequently, production of recombinant spider silk proteins (spidroins) in more amenable hosts is an exciting field of research. For large-scale production to be viable, a heterologous silk production system that is both highly efficient and cost effective is essential. Genes encoding recombinant spidroin have been expressed in bacterial, yeast, insect, and mammalian cells, in addition to many other platforms. This review discusses the recent advances in exploiting an increasingly diverse range of host platforms in the heterologous production of recombinant spidroins.
Identifiants
pubmed: 33051051
pii: S0167-7799(20)30244-4
doi: 10.1016/j.tibtech.2020.09.007
pii:
doi:
Substances chimiques
Recombinant Proteins
0
Silk
0
Fibroins
9007-76-5
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
560-573Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/M017702/1
Pays : United Kingdom
Informations de copyright
Copyright © 2020 The Authors. Published by Elsevier Ltd.. All rights reserved.