Structural and functional characterization of engineered bifunctional fusion proteins of CD39 and CD73 ectonucleotidases.
5'-Nucleotidase
/ chemistry
Adenine Nucleotides
/ metabolism
Anti-Inflammatory Agents
/ chemistry
Apyrase
/ chemistry
GPI-Linked Proteins
/ chemistry
HEK293 Cells
Humans
Hydrogen-Ion Concentration
Hydrolysis
Kinetics
Platelet Aggregation
/ drug effects
Platelet Aggregation Inhibitors
/ chemistry
Protein Conformation
Protein Engineering
Recombinant Fusion Proteins
/ metabolism
Signal Transduction
Structure-Activity Relationship
Substrate Specificity
bifunctional fusion
ectonucleotidase
inflammation
platelet aggregation
purinergic signaling
Journal
American journal of physiology. Cell physiology
ISSN: 1522-1563
Titre abrégé: Am J Physiol Cell Physiol
Pays: United States
ID NLM: 100901225
Informations de publication
Date de publication:
01 01 2021
01 01 2021
Historique:
pubmed:
15
10
2020
medline:
9
2
2021
entrez:
14
10
2020
Statut:
ppublish
Résumé
Extracellular diphosphate and triphosphate nucleotides are released from activated or injured cells to trigger vascular and immune P
Identifiants
pubmed: 33052071
doi: 10.1152/ajpcell.00430.2020
pmc: PMC7846972
doi:
Substances chimiques
Adenine Nucleotides
0
Anti-Inflammatory Agents
0
GPI-Linked Proteins
0
Platelet Aggregation Inhibitors
0
Recombinant Fusion Proteins
0
5'-Nucleotidase
EC 3.1.3.5
NT5E protein, human
EC 3.1.3.5
Apyrase
EC 3.6.1.5
ENTPD1 protein, human
EC 3.6.1.5
Types de publication
Comparative Study
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
C15-C29Subventions
Organisme : HHS | NIH | National Cancer Institute (NCI)
ID : CA221702
Pays : International
Organisme : NCI NIH HHS
ID : R21 CA221702
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK108894
Pays : United States
Organisme : NICHD NIH HHS
ID : R01 HD098363
Pays : United States
Organisme : HHS | NIH | National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
ID : DK108894
Pays : International
Organisme : NIGMS NIH HHS
ID : R35 GM136429
Pays : United States
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