Intra-hospital dissemination of clinical and environmental isolates of Stenotrophomonas maltophilia from Tehran.
Stenotrophomonas maltophilia
Gram-negative bacilli
clinic
environment
hospital
pulsed field gel electrophoresis
Journal
Letters in applied microbiology
ISSN: 1472-765X
Titre abrégé: Lett Appl Microbiol
Pays: England
ID NLM: 8510094
Informations de publication
Date de publication:
Mar 2021
Mar 2021
Historique:
received:
25
06
2020
revised:
11
10
2020
accepted:
12
10
2020
pubmed:
17
10
2020
medline:
14
4
2021
entrez:
16
10
2020
Statut:
ppublish
Résumé
Stenotrophomonas maltophilia isolates are responsible for various hospital-acquired infections and are particularly increasing in the immunocompromised patients. The aim of this study was to determine the clonal relatedness between S. maltophilia isolates originating from the clinic and environment. A total of 150 S. maltophilia isolates from patients and 1108 environmental samples obtained in three hospitals from Tehran. Following molecular identification targeting 23S rRNA gene, the clonal relatedness of the environmental and clinical isolates was determined using pulsed field gel electrophoresis (PFGE). Of the 150 clinical and 18 environmental isolates identified using phenotypic tests, the speciation of 120 and 15 was confirmed by targeting the 23S rRNA gene. The 24 common pulsotypes (PTs) and 32 single PTs were identified by PFGE. Only a small cluster was shared among the clinic and environment within a hospital; therefore, the intra-hospital dissemination of certain isolates of S. maltophilia among the clinic and environment was demonstrated.
Substances chimiques
RNA, Ribosomal, 23S
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
325-331Subventions
Organisme : Tehran University of Medical Sciences and Health Services
ID : 14992
Informations de copyright
© 2020 The Society for Applied Microbiology.
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