CRISPR/Cas9-mediated gene knockout in human adipose stem/progenitor cells.
Adipose stem cells
CRISPR/Cas9
ageing
genome editing
loss-of-function
obesity
Journal
Adipocyte
ISSN: 2162-397X
Titre abrégé: Adipocyte
Pays: United States
ID NLM: 101567863
Informations de publication
Date de publication:
12 2020
12 2020
Historique:
entrez:
19
10
2020
pubmed:
20
10
2020
medline:
17
7
2021
Statut:
ppublish
Résumé
The CRISPR/Cas9 system is a powerful tool to generate a specific loss-of-function phenotype by gene knockout (KO). However, this approach is challenging in primary human cells. In this technical report, we present a reliable protocol to achieve a functional KO in the genome of human adipose stem/progenitor cells (ASCs). Using Sprouty1 (
Identifiants
pubmed: 33070670
doi: 10.1080/21623945.2020.1834230
pmc: PMC7575003
doi:
Substances chimiques
Biomarkers
0
RNA, Small Interfering
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
626-635Références
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