Characterising the immune microenvironment in liposarcoma, its impact on prognosis and the impact of radiotherapy.
Adolescent
Adult
Aged
CD8-Positive T-Lymphocytes
/ immunology
Female
Follow-Up Studies
Humans
Liposarcoma
/ immunology
Lymphocytes, Tumor-Infiltrating
/ immunology
Male
Middle Aged
Neoplasm Recurrence, Local
/ immunology
Prognosis
Radiotherapy
/ methods
Retrospective Studies
Tumor Microenvironment
/ immunology
Young Adult
liposarcoma
lymphocytes
tumour microenvironment
tumour-infiltrating
Journal
Journal of surgical oncology
ISSN: 1096-9098
Titre abrégé: J Surg Oncol
Pays: United States
ID NLM: 0222643
Informations de publication
Date de publication:
Jan 2021
Jan 2021
Historique:
received:
21
07
2020
revised:
12
09
2020
accepted:
21
09
2020
pubmed:
22
10
2020
medline:
12
1
2021
entrez:
21
10
2020
Statut:
ppublish
Résumé
Limited literature exists examining the immune microenvironment in liposarcoma, particularly with regard to the impact of radiotherapy. A major problem is the lack of scoring system for the tumour-infiltrating lymphocytes (TILs) in sarcoma. This study aims to describe the immune environment pre- and postradiotherapy and identify the optimal immune infiltrate scoring system for sarcoma. Thirty-nine paired tissue samples (pre- and postradiotherapy) from patients with liposarcoma were scored by two pathologists for TILs using pre-existing systems (for breast cancer and melanoma) and compared for interobserver reliability. Immunohistochemical staining was performed for various immune markers. The TIL scoring system for breast cancer yielded perfect agreement (κ = 1.000). 21% of patients had increased TILs after radiotherapy, 87.5% of whom had dedifferentiated liposarcoma. Immune suppressor expression was increased frequently after radiotherapy (CD68 increased in 59.4%, PD-L1 increased in 25%). Immune effector expression (CD8) was unchanged in 84.4%. Breast cancer TIL scoring is reproducible in liposarcoma and has high interobserver reliability. Radiotherapy was observed to have a limited impact on immune effectors but seemed to have more impact in upregulating immune suppressors, suggesting radiotherapy may contribute to disease control through immunomodulatory effects. Dedifferentiated liposarcoma represents a uniquely responsive subtype.
Sections du résumé
BACKGROUND AND OBJECTIVES
OBJECTIVE
Limited literature exists examining the immune microenvironment in liposarcoma, particularly with regard to the impact of radiotherapy. A major problem is the lack of scoring system for the tumour-infiltrating lymphocytes (TILs) in sarcoma. This study aims to describe the immune environment pre- and postradiotherapy and identify the optimal immune infiltrate scoring system for sarcoma.
METHODS
METHODS
Thirty-nine paired tissue samples (pre- and postradiotherapy) from patients with liposarcoma were scored by two pathologists for TILs using pre-existing systems (for breast cancer and melanoma) and compared for interobserver reliability. Immunohistochemical staining was performed for various immune markers.
RESULTS
RESULTS
The TIL scoring system for breast cancer yielded perfect agreement (κ = 1.000). 21% of patients had increased TILs after radiotherapy, 87.5% of whom had dedifferentiated liposarcoma. Immune suppressor expression was increased frequently after radiotherapy (CD68 increased in 59.4%, PD-L1 increased in 25%). Immune effector expression (CD8) was unchanged in 84.4%.
CONCLUSIONS
CONCLUSIONS
Breast cancer TIL scoring is reproducible in liposarcoma and has high interobserver reliability. Radiotherapy was observed to have a limited impact on immune effectors but seemed to have more impact in upregulating immune suppressors, suggesting radiotherapy may contribute to disease control through immunomodulatory effects. Dedifferentiated liposarcoma represents a uniquely responsive subtype.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
117-126Subventions
Organisme : Hannah's Chance Foundation
ID : 35 (2017)
Informations de copyright
© 2020 Wiley Periodicals LLC.
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