Cytidine Monophosphate
Animals
Capsid Proteins
/ genetics
Cell Line
Cell Membrane
/ metabolism
Cell Survival
Mice
N-Acetylneuraminic Acid
/ metabolism
N-Acylneuraminate Cytidylyltransferase
/ genetics
Nucleotide Transport Proteins
/ genetics
Receptors, Virus
/ metabolism
Reoviridae
/ genetics
Reoviridae Infections
/ metabolism
Virus Attachment
Virus Replication
cytidine monophosphate N-acetylneuraminic acid synthetase
microglia
reovirus
sialic acid
solute carrier family 35 member A1
viral attachment
Journal
Journal of virology
ISSN: 1098-5514
Titre abrégé: J Virol
Pays: United States
ID NLM: 0113724
Informations de publication
Date de publication:
22 12 2020
22 12 2020
Historique:
received:
03
08
2020
accepted:
15
10
2020
pubmed:
23
10
2020
medline:
19
3
2021
entrez:
22
10
2020
Statut:
epublish
Résumé
Engagement of cell surface receptors by viruses is a critical determinant of viral tropism and disease. The reovirus attachment protein σ1 binds sialylated glycans and proteinaceous receptors to mediate infection, but the specific requirements for different cell types are not entirely known. To identify host factors required for reovirus-induced cell death, we conducted a CRISPR-knockout screen targeting over 20,000 genes in murine microglial BV2 cells. Candidate genes required for reovirus to cause cell death were highly enriched for sialic acid synthesis and transport. Two of the top candidates identified, CMP
Identifiants
pubmed: 33087464
pii: JVI.01571-20
doi: 10.1128/JVI.01571-20
pmc: PMC7944449
pii:
doi:
Substances chimiques
Capsid Proteins
0
Nucleotide Transport Proteins
0
Receptors, Virus
0
Slc35a1 protein, mouse
0
sigma 1 protein, reovirus
0
N-Acylneuraminate Cytidylyltransferase
EC 2.7.7.43
N-Acetylneuraminic Acid
GZP2782OP0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIAID NIH HHS
ID : U19 AI109725
Pays : United States
Organisme : NIAID NIH HHS
ID : K08 AI128043
Pays : United States
Organisme : NIAID NIH HHS
ID : U19 AI142784
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI038296
Pays : United States
Organisme : NIDDK NIH HHS
ID : R00 DK116666
Pays : United States
Informations de copyright
Copyright © 2020 American Society for Microbiology.
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