Italian observational study on HPV infection, E6, and p16 expression in men with penile cancer.
Aged
Cyclin-Dependent Kinase Inhibitor p16
/ genetics
DNA, Viral
/ genetics
Hospitals, University
/ statistics & numerical data
Humans
Male
Middle Aged
Oncogene Proteins, Viral
/ genetics
Papillomaviridae
/ classification
Papillomavirus Infections
/ complications
Penile Neoplasms
/ virology
Repressor Proteins
/ genetics
Retrospective Studies
Tertiary Care Centers
/ statistics & numerical data
E6
HPV
HPV-DNA
Penile cancer
p16
Journal
Virology journal
ISSN: 1743-422X
Titre abrégé: Virol J
Pays: England
ID NLM: 101231645
Informations de publication
Date de publication:
22 10 2020
22 10 2020
Historique:
received:
26
08
2020
accepted:
30
09
2020
entrez:
23
10
2020
pubmed:
24
10
2020
medline:
20
8
2021
Statut:
epublish
Résumé
Human Papillomavirus (HPV) infection is one of the most important causes of cancer. It can play a role in cervical and extra-cervical cancers. Penile cancer is rare, even if an increasing trend was recently reported. Aim of the present study was to assess the prevalence and distribution of HPV genotypes in cases of penile cancer diagnosed in Sardinia, Italy. Surrogate markers of HPV infection (i.e., E6 and p16 genes) were also evaluated in all cases. An observational, retrospective study which recruited all cases of penile cancer diagnosed between 2002 and 2019 at a tertiary care hospital in Sardinia, Italy, was carried out. HPV-DNA detection and genotyping were performed by Real-time PCR. Specimens were tested for oncogene E6 mRNA and for p16(INK4a) expression. HPV prevalence was 28.1% (9/32); HPV-16 was the most prevalent genotype (7/9, 77.8%). p16INK4a positivity was found in 66.7% of the samples with a statistically significant difference between HPV-positive and -negative groups. E6-transcript was detected in 71% of the HPV-16 positive samples. The overall survival was not statistically different between HPV-positives and -negatives. The present study confirms the etiologic role of HPV in penile cancer and supports the adoption of vaccination strategies in men and women. Further studies should clarify the diagnostic and prognostic role of E6 and p16 proteins. HPV infection can favor the occurrence of penile cancer, whose diagnosis and prognosis could be improved with the implementation of validated molecular techniques.
Sections du résumé
BACKGROUND
Human Papillomavirus (HPV) infection is one of the most important causes of cancer. It can play a role in cervical and extra-cervical cancers. Penile cancer is rare, even if an increasing trend was recently reported. Aim of the present study was to assess the prevalence and distribution of HPV genotypes in cases of penile cancer diagnosed in Sardinia, Italy. Surrogate markers of HPV infection (i.e., E6 and p16 genes) were also evaluated in all cases.
METHODS
An observational, retrospective study which recruited all cases of penile cancer diagnosed between 2002 and 2019 at a tertiary care hospital in Sardinia, Italy, was carried out. HPV-DNA detection and genotyping were performed by Real-time PCR. Specimens were tested for oncogene E6 mRNA and for p16(INK4a) expression.
RESULTS
HPV prevalence was 28.1% (9/32); HPV-16 was the most prevalent genotype (7/9, 77.8%). p16INK4a positivity was found in 66.7% of the samples with a statistically significant difference between HPV-positive and -negative groups. E6-transcript was detected in 71% of the HPV-16 positive samples. The overall survival was not statistically different between HPV-positives and -negatives.
DISCUSSION
The present study confirms the etiologic role of HPV in penile cancer and supports the adoption of vaccination strategies in men and women. Further studies should clarify the diagnostic and prognostic role of E6 and p16 proteins.
CONCLUSION
HPV infection can favor the occurrence of penile cancer, whose diagnosis and prognosis could be improved with the implementation of validated molecular techniques.
Identifiants
pubmed: 33092608
doi: 10.1186/s12985-020-01424-9
pii: 10.1186/s12985-020-01424-9
pmc: PMC7579865
doi:
Substances chimiques
Cyclin-Dependent Kinase Inhibitor p16
0
DNA, Viral
0
E6 protein, Human papillomavirus type 16
0
Oncogene Proteins, Viral
0
Repressor Proteins
0
Types de publication
Journal Article
Observational Study
Langues
eng
Sous-ensembles de citation
IM
Pagination
161Références
Eur Urol. 2016 May;69(5):953-61
pubmed: 26762611
Int J Environ Res Public Health. 2020 Jun 23;17(12):
pubmed: 32585996
Lancet Oncol. 2019 Jan;20(1):145-158
pubmed: 30573285
Urology. 2010 Aug;76(2 Suppl 1):S2-6
pubmed: 20691882
J Infect Dis. 2006 Oct 15;194(8):1044-57
pubmed: 16991079
JAMA Netw Open. 2018 Sep 7;1(5):e181999
pubmed: 30646145
Gynecol Oncol. 1998 May;69(2):114-21
pubmed: 9600817
Int J Cancer. 2006 Sep 1;119(5):1078-81
pubmed: 16570278
Gynecol Oncol. 2007 May;105(2):351-7
pubmed: 17275890
Cancer Epidemiol Biomarkers Prev. 2018 Oct;27(10):1123-1132
pubmed: 29987099
Lancet Glob Health. 2020 Feb;8(2):e180-e190
pubmed: 31862245
Int J Environ Res Public Health. 2020 Jan 01;17(1):
pubmed: 31906371
Biomed Res Int. 2018 Jul 19;2018:8345893
pubmed: 30105253
CA Cancer J Clin. 2018 Nov;68(6):394-424
pubmed: 30207593