Effects of topical application of tramadol with/without dexmedetomidine and proparacaine on corneal sensitivity in rats.

Corneal anesthesia Dexmedetomidine hydrochloride Esthesiometry Proparacaine hydrochloride Rat Tramadol hydrochloride

Journal

International ophthalmology
ISSN: 1573-2630
Titre abrégé: Int Ophthalmol
Pays: Netherlands
ID NLM: 7904294

Informations de publication

Date de publication:
Feb 2021
Historique:
received: 15 05 2020
accepted: 01 10 2020
pubmed: 24 10 2020
medline: 22 6 2021
entrez: 23 10 2020
Statut: ppublish

Résumé

To evaluate the corneal anesthetic effect following topical application of tramadol alone and in combination with dexmedetomidine, and compare it to proparacaine, in clinically healthy rats. A randomized, crossover study was performed. Twenty Wistar albino rats (n = 40 eyes) were used. Corneal touch threshold (CTT) measurements (in mm) were obtained using a Cochet-Bonnet aesthesiometer. CTT measurements were obtained at baseline, 1-min following application of the topical anesthetic agent, and repeated at 5-min intervals up to 75 min. The topical protocol involved 3 treatment conditions, separated by a 2-week washout period: proparacaine, tramadol alone, and tramadol in combination with dexmedetomidine. CTT values were significantly decreased compared to baseline at each timepoint until completion of the 75-min evaluation in all treated eyes, regardless of the assigned treatment (p < 0.0083). With tramadol, complete corneal anesthesia (CTT = 0) was achieved within 1-5 min in 18 eyes and ranged from 5 to 25 min. Co-administration of dexmedetomidine to tramadol resulted in significantly increased CTT values from 5 to 20 min following topical application, compared to tramadol alone (p < 0.0083), and complete corneal anesthesia was achieved in only 14 out of 20 treated eyes. Tramadol might be a useful alternative to topical anesthetic agents, providing a dose-related corneal anesthetic effect. Co-administration of dexmedetomidine does not potentiate its anesthetic effect. The underlying mechanism(s) of drug antagonism between tramadol and dexmedetomidine remains to be determined.

Identifiants

pubmed: 33095346
doi: 10.1007/s10792-020-01596-x
pii: 10.1007/s10792-020-01596-x
doi:

Substances chimiques

Anesthetics, Local 0
Dexmedetomidine 67VB76HONO
Ophthalmic Solutions 0
Propoxycaine EPD1EH7F53
proxymetacaine B4OB0JHI1X
Tramadol 39J1LGJ30J

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

465-473

Références

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Auteurs

Cristina A Lelescu (CA)

Modis Competence Center, Modis Life Sciences Belgium, Mureșului 9, 400000, Cluj-Napoca, Romania.

Daria A Dumitras (DA)

Department of Biochemistry, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, Calea Mănăştur 3-5, 400372, Cluj-Napoca, Romania.

Sonia Iurian (S)

Department of Pharmaceutical Technology and Biopharmaceutics, Faculty of Pharmacy, University of Medicine and Pharmacy "Iuliu Hațieganu", V. Babeș 41, 400012, Cluj-Napoca, Romania.

Francesco Staffieri (F)

Division of Veterinary Surgery, Department of Emergency and Organ Transplantation, Faculty of Veterinary Medicine, University "Aldo Moro" of Bari, SP Per Casamassima Km. 3, 70010, Valenzano, Bari, Italy.

Cosmin Muresan (C)

Department of Surgery, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, Calea Mănăştur 3-5, 400372, Cluj-Napoca, Romania. muresanscosmin@gmail.com.

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