Micropatterned soft hydrogels to study the interplay of receptors and forces in T cell activation.
APC model
T cell mechanotransduction
immunomodulatory interfaces
micropatterned hydrogels for immunology
Journal
Acta biomaterialia
ISSN: 1878-7568
Titre abrégé: Acta Biomater
Pays: England
ID NLM: 101233144
Informations de publication
Date de publication:
01 01 2021
01 01 2021
Historique:
received:
26
01
2020
revised:
28
09
2020
accepted:
15
10
2020
pubmed:
26
10
2020
medline:
15
5
2021
entrez:
25
10
2020
Statut:
ppublish
Résumé
The analysis of T cell responses to mechanical properties of antigen presenting cells (APC) is experimentally challenging at T cell-APC interfaces. Soft hydrogels with adjustable mechanical properties and biofunctionalization are useful reductionist models to address this problem. Here, we report a methodology to fabricate micropatterned soft hydrogels with defined stiffness to form spatially confined T cell/hydrogel contact interfaces at micrometer scale. Using automatized microcontact printing we prepared arrays of anti-CD3 microdots on poly(acrylamide) hydrogels with Young's Modulus in the range of 2 to 50 kPa. We optimized the printing process to obtain anti-CD3 microdots with constant area (50 µm
Identifiants
pubmed: 33099024
pii: S1742-7061(20)30617-6
doi: 10.1016/j.actbio.2020.10.028
pii:
doi:
Substances chimiques
Hydrogels
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
234-246Informations de copyright
Copyright © 2020. Published by Elsevier Ltd.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.