T cell-derived exosomes induced macrophage inflammatory protein-1α/β drive the trafficking of CD8


Journal

Journal of cellular and molecular medicine
ISSN: 1582-4934
Titre abrégé: J Cell Mol Med
Pays: England
ID NLM: 101083777

Informations de publication

Date de publication:
12 2020
Historique:
received: 10 07 2020
revised: 22 09 2020
accepted: 06 10 2020
pubmed: 28 10 2020
medline: 13 5 2021
entrez: 27 10 2020
Statut: ppublish

Résumé

Oral lichen planus (OLP) is a T cell-mediated chronic inflammatory disease with uncertain aetiology. Exosomes are nanosized particles with biological capacities. Here, we aimed to study the effects of T cell-derived exosomes (T-exos) on the pathogenesis of OLP and its mechanism. T-exos were incubated with Jurkat cells for 48 hours, and 26 cytokines in the supernatant were measured by luminex assay. The expression of macrophage inflammatory protein (MIP)-1α/β was detected using immunohistochemistry and ELISA; that of CCR1/3/5 on peripheral T cells was determined by flow cytometry. Transwell assay was performed to investigate the chemotactic effect of MIP-1α/β, and cells in the lower chambers were examinated by flow cytometry. As a result, OLP T-exos elevated the production of MIP-1α/β, which were highly expressed in OLP tissues and plasma. CCR1/5 were markedly expressed on OLP peripheral T cells, and the majority of CCR1/5

Identifiants

pubmed: 33107682
doi: 10.1111/jcmm.16020
pmc: PMC7754044
doi:

Substances chimiques

Biomarkers 0
Chemokine CCL3 0
Chemokine CCL4 0
Cytokines 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

14086-14098

Informations de copyright

© 2020 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd.

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Auteurs

Jing-Ya Yang (JY)

The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, China.

Jing Zhang (J)

The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, China.
Department of Oral Medicine, School and Hospital of Stomatology, Wuhan University, Wuhan, China.

Rui Lu (R)

Department of Oral Medicine, School and Hospital of Stomatology, Wuhan University, Wuhan, China.

Ya-Qin Tan (YQ)

The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, China.
Department of Oral Medicine, School and Hospital of Stomatology, Wuhan University, Wuhan, China.

Ge-Fei Du (GF)

Department of Oral Medicine, School and Hospital of Stomatology, Wuhan University, Wuhan, China.

Gang Zhou (G)

The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, China.
Department of Oral Medicine, School and Hospital of Stomatology, Wuhan University, Wuhan, China.

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