Efficient extraction and preservation of thermotolerant phycocyanins from red alga Cyanidioschyzon merolae.

Hot Temperature Hydrogen-Ion Concentration Phycocyanin / isolation & purification Rhodophyta / enzymology Thermotolerance
Cyanidioschyzon merolae Microalgae Phycocyanin Protein extraction Red algae

Journal

Journal of bioscience and bioengineering
ISSN: 1347-4421
Titre abrégé: J Biosci Bioeng
Pays: Japan
ID NLM: 100888800

Informations de publication

Date de publication:
Feb 2021
Historique:
received: 01 06 2020
revised: 21 09 2020
accepted: 29 09 2020
pubmed: 29 10 2020
medline: 25 2 2021
entrez: 28 10 2020
Statut: ppublish

Résumé

C-Phycocyanin (PC) is a protein used commercially as a natural blue pigment produced by cyanobacteria, cryptophytes, and rhodophytes. Although it is industrially synthesized from the cyanobacterium Arthrospira platensis, PC requires high levels of energy for its extraction, which involves freezing of cells. However, as a protein, PC is easily denatured at extreme temperatures. In this study, we extracted PC from the red alga Cyanidioschyzon merolae, denoted as CmPC, and found that this protein was tolerant to high temperatures and acidic pH. CmPC was extracted by suspending cells in water mixed with various salts and organic acids without freeze-drying or freeze-thaw. The stability of CmPC varied with salt concentration and was destabilized by organic acids. Our results indicate that C. merolae is a potential candidate for PC production with thermotolerant properties.

Identifiants

DOI: 10.1016/j.jbiosc.2020.09.021 PMID: 33109478
pubmed: 33109478
pii: S1389-1723(20)30370-4
doi: 10.1016/j.jbiosc.2020.09.021
pii:
doi:

Substances chimiques

Phycocyanin 11016-15-2

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

161-167

Informations de copyright

Copyright © 2020 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

Auteurs

Chihiro Yoshida (C)

School of Agriculture, Meiji University, 1-1-1 Higashimita, Tama-ku, Kawasaki, Kanagawa 214-8571, Japan.

Miyabi Murakami (M)

School of Agriculture, Meiji University, 1-1-1 Higashimita, Tama-ku, Kawasaki, Kanagawa 214-8571, Japan.

Anna Niwa (A)

School of Agriculture, Meiji University, 1-1-1 Higashimita, Tama-ku, Kawasaki, Kanagawa 214-8571, Japan.

Masahiro Takeya (M)

School of Agriculture, Meiji University, 1-1-1 Higashimita, Tama-ku, Kawasaki, Kanagawa 214-8571, Japan.

Takashi Osanai (T)

School of Agriculture, Meiji University, 1-1-1 Higashimita, Tama-ku, Kawasaki, Kanagawa 214-8571, Japan. Electronic address: tosanai@meiji.ac.jp.

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Classifications MeSH

questionsmedicales.fr › Environnement et santé publique › Environnement › Concepts météorologiques › Atmosphère › Temps (météorologie) › Température › Température élevée › Efficient extraction and preservation of...
questionsmedicales.fr › Phénomènes chimiques › Concentration en ions d'hydrogène › Efficient extraction and preservation of...
questionsmedicales.fr › Facteurs biologiques › Pigments biologiques › Phycocyanine › Efficient extraction and preservation of...
questionsmedicales.fr › Eucaryotes › Rhodophyta › Efficient extraction and preservation of...
questionsmedicales.fr › Phénomènes biologiques › Adaptation biologique › Adaptation physiologique › Acclimatation › Thermotolérance › Efficient extraction and preservation of...