Search for viral agents in cerebrospinal fluid in patients with multiple sclerosis using real-time PCR and metagenomics.
Adolescent
Adult
Aged
Autoimmune Diseases
/ immunology
Enterovirus
/ isolation & purification
Female
Herpesvirus 3, Human
/ isolation & purification
Herpesvirus 4, Human
/ isolation & purification
Herpesvirus 6, Human
/ isolation & purification
Humans
Male
Metagenomics
Middle Aged
Multiple Sclerosis
/ genetics
Myelin Sheath
/ genetics
Real-Time Polymerase Chain Reaction
Virus Diseases
/ genetics
Young Adult
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2020
2020
Historique:
received:
29
03
2020
accepted:
29
09
2020
entrez:
28
10
2020
pubmed:
29
10
2020
medline:
15
12
2020
Statut:
epublish
Résumé
Multiple sclerosis (MS) is a chronic, immune-mediated demyelinating disease of the central nervous system of unclear etiology, but there is some evidence that viral infections could be responsible for triggering autoimmune mechanisms against myelin. We searched for viral RNA and DNA in cerebrospinal fluid (CSF) of 34 MS patients and 13 controls using RT-PCR/PCR against common neurotropic viruses. In addition, shotgun DNA- and RNA-based metagenomics were done in 13 MS patients and 4 controls. Specific quantitative real-time RT-PCR/PCR testing revealed the presence of viral nucleic acid in seven (20.59%) MS patients and in one (7.69%) control patient. In MS patients the most frequently detected was human herpesvirus type 6 (HHV-6; 3 cases; 8.82%); followed by Epstein-Barr virus (EBV; 2 cases; 5.88%), varicella zoster virus (VZV; 1 case; 2.94%) and Enterovirus (EV; 1 case; 2.94%). The single identified virus among controls was EBV (7.69%). DNA and RNA metagenomic assays did not identify any known eukaryotic viruses even though three of the analyzed samples were low-level positive by specific quantitative real-time PCR. In conclusion, we detected the presence of Herpesviridae and occasionally Enteroviridae in CSF from patients with MS but their prevalence was not significantly higher than among controls. Metagenomic analysis seems to be less sensitive than real-time RT-PCR/PCR and it did not detect any potential viral pathogens.
Identifiants
pubmed: 33112911
doi: 10.1371/journal.pone.0240601
pii: PONE-D-20-08960
pmc: PMC7592794
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0240601Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
Int J Mol Sci. 2012;13(9):11718-52
pubmed: 23109880
Lancet Neurol. 2018 Feb;17(2):162-173
pubmed: 29275977
J Microbiol Immunol Infect. 2014 Oct;47(5):418-21
pubmed: 23731901
Arch Neurol. 2007 Jan;64(1):72-5
pubmed: 17210811
Med Dosw Mikrobiol. 2010;62(3):245-53
pubmed: 21114017
Mult Scler. 1998 Dec;4(6):490-6
pubmed: 9987758
Autoimmun Rev. 2002 Oct;1(5):251-60
pubmed: 12848977
J Neurovirol. 2007 Aug;13(4):347-52
pubmed: 17849318
Curr Opin Virol. 2014 Dec;9:127-33
pubmed: 25462444
Arch Immunol Ther Exp (Warsz). 2015 Feb;63(1):79-84
pubmed: 25376263
PLoS Pathog. 2014 Nov 20;10(11):e1004437
pubmed: 25412476
Curr Neuropharmacol. 2011 Sep;9(3):409-16
pubmed: 22379455
Ann Neurol. 2007 Jun;61(6):504-13
pubmed: 17492755
Adv Exp Med Biol. 2016;935:89-98
pubmed: 27311319
J Virol. 2002 Jun;76(12):6147-54
pubmed: 12021348
Emerg Infect Dis. 2013;19(9):
pubmed: 23968557
J Infect Dis. 2017 Mar 15;215(6):896-901
pubmed: 28453842
Neurology. 2000 Jul 25;55(2):178-84
pubmed: 10908887
Biochim Biophys Acta. 2011 Feb;1812(2):132-40
pubmed: 20600868
Bioinformatics. 2014 Aug 1;30(15):2114-20
pubmed: 24695404
Clin Neurol Neurosurg. 2014 Apr;119:44-8
pubmed: 24635924
Acta Neuropathol. 1989;77(3):333-5
pubmed: 2922996
Mult Scler. 2008 Jan;14(1):54-8
pubmed: 17893113
J Med Econ. 2013;16(5):639-47
pubmed: 23425293
Ann Neurol. 2011 Feb;69(2):292-302
pubmed: 21387374
CNS Neurol Disord Drug Targets. 2012 Aug;11(5):528-44
pubmed: 22583435
Med Dosw Mikrobiol. 2012;64(2):139-49
pubmed: 23072059
Immunology. 2019 Jan;156(1):9-22
pubmed: 30222193
Brain Pathol. 2001 Jan;11(1):92-106
pubmed: 11145206
Genes (Basel). 2019 Aug 29;10(9):
pubmed: 31470675
PLoS One. 2012;7(3):e31886
pubmed: 22412845
PLoS One. 2010 Sep 01;5(9):
pubmed: 20824132
Adv Exp Med Biol. 2016 Jul 13;:
pubmed: 27405447
Nat Methods. 2012 Mar 04;9(4):357-9
pubmed: 22388286
J Virol Methods. 2015 Dec 15;226:1-6
pubmed: 26424618
Eur J Paediatr Neurol. 2015 Mar;19(2):266-70
pubmed: 25576193
Future Virol. 2010 May;5(3):313-323
pubmed: 21731577
Microbiome. 2017 May 5;5(1):52
pubmed: 28476139
Ther Adv Neurol Disord. 2013 Mar;6(2):81-116
pubmed: 23483715
Ann Neurol. 2003 Feb;53(2):189-97
pubmed: 12557285
Med Dosw Mikrobiol. 2008;60(3):259-65
pubmed: 19143180
Neurology. 2009 Jul 7;73(1):32-8
pubmed: 19458321
Science. 2019 Sep 27;365(6460):
pubmed: 31604244
Genome Res. 2011 Jun;21(6):936-9
pubmed: 20980556
Bioinformatics. 2009 Aug 15;25(16):2078-9
pubmed: 19505943
Mult Scler. 2008 Jun;14(5):595-601
pubmed: 18566025
Ther Adv Neurol Disord. 2012 Jan;5(1):13-22
pubmed: 22276073
Nucleic Acids Res. 2013 Jan 7;41(1):e13
pubmed: 22962364
Neurosciences (Riyadh). 2010 Jan;15(1):46-8
pubmed: 20677593
Genes (Basel). 2019 Apr 30;10(5):
pubmed: 31052348
Lancet Neurol. 2019 Mar;18(3):269-285
pubmed: 30679040
Ann Neurol. 2006 Mar;59(3):499-503
pubmed: 16502434
Front Cell Infect Microbiol. 2017 Jan 06;6:198
pubmed: 28111617
BMC Biol. 2014 Nov 12;12:87
pubmed: 25387460