Comprehensive screening of genomic and metagenomic data reveals a large diversity of tetracycline resistance genes.
Anti-Bacterial Agents
/ pharmacology
Escherichia coli
/ drug effects
Gene Transfer, Horizontal
/ genetics
Humans
Interspersed Repetitive Sequences
/ genetics
Membrane Transport Proteins
/ genetics
Metagenome
/ genetics
Phylogeny
Ribosomal Proteins
/ genetics
Tetracycline
/ pharmacology
Tetracycline Resistance
/ genetics
antibiotic resistance
hidden Markov model
metagenomics
microbiome
resistome
tetracycline resistance
Journal
Microbial genomics
ISSN: 2057-5858
Titre abrégé: Microb Genom
Pays: England
ID NLM: 101671820
Informations de publication
Date de publication:
11 2020
11 2020
Historique:
pubmed:
31
10
2020
medline:
17
8
2021
entrez:
30
10
2020
Statut:
ppublish
Résumé
Tetracyclines are broad-spectrum antibiotics used to prevent or treat a variety of bacterial infections. Resistance is often mediated through mobile resistance genes, which encode one of the three main mechanisms: active efflux, ribosomal target protection or enzymatic degradation. In the last few decades, a large number of new tetracycline-resistance genes have been discovered in clinical settings. These genes are hypothesized to originate from environmental and commensal bacteria, but the diversity of tetracycline-resistance determinants that have not yet been mobilized into pathogens is unknown. In this study, we aimed to characterize the potential tetracycline resistome by screening genomic and metagenomic data for novel resistance genes. By using probabilistic models, we predicted 1254 unique putative tetracycline resistance genes, representing 195 gene families (<70 % amino acid sequence identity), whereof 164 families had not been described previously. Out of 17 predicted genes selected for experimental verification, 7 induced a resistance phenotype in an
Identifiants
pubmed: 33125315
doi: 10.1099/mgen.0.000455
pmc: PMC7725328
doi:
Substances chimiques
Anti-Bacterial Agents
0
Membrane Transport Proteins
0
Ribosomal Proteins
0
Tetracycline
F8VB5M810T
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
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