Development a multiplex RT-PCR assay for simultaneous detection of African swine fever virus, classical swine fever virus and atypical porcine pestivirus.
African swine fever virus (ASFV)
Atypical porcine pestivirus (APPV)
Classical swine fever virus (CSFV)
Multiplex RT-PCR (mRT-PCR)
Journal
Journal of virological methods
ISSN: 1879-0984
Titre abrégé: J Virol Methods
Pays: Netherlands
ID NLM: 8005839
Informations de publication
Date de publication:
01 2021
01 2021
Historique:
received:
18
07
2020
revised:
19
10
2020
accepted:
20
10
2020
pubmed:
1
11
2020
medline:
25
11
2021
entrez:
31
10
2020
Statut:
ppublish
Résumé
African swine fever virus (ASFV), classical swine fever virus (CSFV) and atypical porcine pestivirus (APPV) have caused considerable financial losses to the pig industry worldwide, and it is critical to achieve early and accurate diagnosis of these viruses to control the diseases induced by them. In this study, three pairs of specific primers were designed based on the highly conserved genome regions of these viruses, and a multiplex reverse transcription-polymerase chain reaction (mRT-PCR) assay for ASFV, CSFV and APPV was established after various reaction conditions were optimized. The mRT-PCR assay consisted of two steps, that is, reverse transcription (RT) and mPCR. The assay was highly specific, sensitive, and reproducible for ASFV, CSFV and APPV without cross-reaction with other swine pathogens. The sensitivity of this assay, which used purified plasmid constructs containing specific viral target fragments as templates, was 6.34 × 10
Identifiants
pubmed: 33127443
pii: S0166-0934(20)30258-5
doi: 10.1016/j.jviromet.2020.114006
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
114006Informations de copyright
Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.