Developing Liquid Rhizobium Inoculants with Enhanced Long-Term Survival, Storage Stability, and Plant Growth Promotion Using Ectoine Additive.
Journal
Current microbiology
ISSN: 1432-0991
Titre abrégé: Curr Microbiol
Pays: United States
ID NLM: 7808448
Informations de publication
Date de publication:
Jan 2021
Jan 2021
Historique:
received:
25
08
2020
accepted:
19
10
2020
pubmed:
3
11
2020
medline:
15
5
2021
entrez:
2
11
2020
Statut:
ppublish
Résumé
Liquid microbial inoculants have recently received great attention due to their vital roles for sustainable agricultural practices. However, long-term conservation under ambient temperature conditions and deleterious environmental factors might negatively impact microbial cell survival and limit their efficacy in the field. Thus, developing efficient liquid formulation providing prolonged survival of rhizobia in the final product and after an application is crucial. Therefore, this study investigates the effect of various additives on the long-term survival of rhizobia stored in liquid cultures at room temperature (25 °C) for 12 months. Various yeast sucrose media amended with polyvinylpyrrolidone (PVP) or gum arabic as colloidal agents in combination with ectoine (as a compatible solute) and/or glycerol were evaluated. A dramatic decline in viable cell count was obtained in formulas amended only with PVP from Log 8.5 to Log 5 in the first six months and then to Log 1.5 after 12 months. In contrast, rhizobia stored at PVP-based formulas amended with 10 mg L
Identifiants
pubmed: 33136205
doi: 10.1007/s00284-020-02265-z
pii: 10.1007/s00284-020-02265-z
doi:
Substances chimiques
Amino Acids, Diamino
0
Culture Media
0
ectoine
7GXZ3858RY
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
282-291Références
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