Membrane progesterone receptor induces meiosis in Xenopus oocytes through endocytosis into signaling endosomes and interaction with APPL1 and Akt2.
Adaptor Proteins, Signal Transducing
/ metabolism
Animals
Endocytosis
Endosomes
/ metabolism
Female
Meiosis
/ physiology
Oocytes
/ metabolism
Progesterone
/ pharmacology
Proto-Oncogene Proteins c-akt
/ metabolism
Receptors, G-Protein-Coupled
/ metabolism
Receptors, Progesterone
/ metabolism
Signal Transduction
Xenopus Proteins
/ metabolism
Xenopus laevis
Journal
PLoS biology
ISSN: 1545-7885
Titre abrégé: PLoS Biol
Pays: United States
ID NLM: 101183755
Informations de publication
Date de publication:
11 2020
11 2020
Historique:
received:
18
12
2019
accepted:
18
09
2020
revised:
12
11
2020
pubmed:
3
11
2020
medline:
5
1
2021
entrez:
2
11
2020
Statut:
epublish
Résumé
The steroid hormone progesterone (P4) mediates many physiological processes through either nuclear receptors that modulate gene expression or membrane P4 receptors (mPRs) that mediate nongenomic signaling. mPR signaling remains poorly understood. Here we show that the topology of mPRβ is similar to adiponectin receptors and opposite to that of G-protein-coupled receptors (GPCRs). Using Xenopus oocyte meiosis as a well-established physiological readout of nongenomic P4 signaling, we demonstrate that mPRβ signaling requires the adaptor protein APPL1 and the kinase Akt2. We further show that P4 induces clathrin-dependent endocytosis of mPRβ into signaling endosome, where mPR interacts transiently with APPL1 and Akt2 to induce meiosis. Our findings outline the early steps involved in mPR signaling and expand the spectrum of mPR signaling through the multitude of pathways involving APPL1.
Identifiants
pubmed: 33137110
doi: 10.1371/journal.pbio.3000901
pii: PBIOLOGY-D-19-03653
pmc: PMC7660923
doi:
Substances chimiques
Adaptor Proteins, Signal Transducing
0
Appl1 protein, Xenopus
0
Receptors, G-Protein-Coupled
0
Receptors, Progesterone
0
Xenopus Proteins
0
progesterone receptor B
0
Progesterone
4G7DS2Q64Y
Proto-Oncogene Proteins c-akt
EC 2.7.11.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e3000901Commentaires et corrections
Type : ErratumIn
Déclaration de conflit d'intérêts
I have read the journal's policy and the authors of this manuscript have the following competing interests: KM is a co-founder of Valdia Health.
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