The Effect of Porphyromonas gingivalis Lipopolysaccharide on the Pyroptosis of Gingival Fibroblasts.
Animals
Caspase 1
/ metabolism
Cells, Cultured
Cytokines
/ genetics
Disease Models, Animal
Female
Fibroblasts
/ drug effects
Gingiva
/ drug effects
Gingivitis
/ chemically induced
Humans
Lipopolysaccharides
/ isolation & purification
Male
Porphyromonas gingivalis
/ metabolism
Pyroptosis
/ drug effects
Rats, Sprague-Dawley
Signal Transduction
Up-Regulation
Porphyromonas gingivalis
fibroblast
lipopolysaccharide
programmed cell death
pyroptosis
Journal
Inflammation
ISSN: 1573-2576
Titre abrégé: Inflammation
Pays: United States
ID NLM: 7600105
Informations de publication
Date de publication:
Jun 2021
Jun 2021
Historique:
received:
26
08
2020
accepted:
27
10
2020
revised:
14
10
2020
pubmed:
4
11
2020
medline:
15
12
2021
entrez:
3
11
2020
Statut:
ppublish
Résumé
Periodontitis is a chronic inflammatory disease induced by Porphyromonas gingivalis (P. gingivalis) and other pathogens. P. gingivalis release various virulence factors including lipopolysaccharide (LPS). However, whether P. gingivalis-LPS inducing pyroptosis in human gingival fibroblasts (HGFs) remains unknown. In present study, P. gingivalis-LPS decreased the membrane integrity of HGFs, and pyroptosis-associated cytokines were upregulated at the mRNA level. In addition, pyroptosis proteins were highly expressed in gingival tissues of periodontitis. P. gingivalis-LPS induced gingivitis in the rat model, and the expression level of pyroptosis-associated proteins increased. Together, P. gingivalis-LPS can activate the pyroptosis reaction, which may be a pro-pyroptosis status in a relative low concentration.
Identifiants
pubmed: 33140204
doi: 10.1007/s10753-020-01379-7
pii: 10.1007/s10753-020-01379-7
doi:
Substances chimiques
Cytokines
0
Lipopolysaccharides
0
Caspase 1
EC 3.4.22.36
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
846-858Subventions
Organisme : Department of Finance of Jilin Province
ID : JCSZ2019378-3
Organisme : Jilin Scientific and Technological Development Program
ID : 20180101121JC
Organisme : Jilin Scientific and Technological Development Program
ID : 20200404108YY
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