Evaluation and comparison of the commonly used bioassays of human indoleamine 2,3-dioxygenase 1 (IDO1) and tryptophan 2,3-dioxygenase (TDO).

Biological Assay Dose-Response Relationship, Drug Enzyme Inhibitors / chemical synthesis HEK293 Cells HeLa Cells Humans Indoleamine-Pyrrole 2,3,-Dioxygenase / antagonists & inhibitors Molecular Structure Recombinant Proteins / metabolism Structure-Activity Relationship Tryptophan Oxygenase / antagonists & inhibitors
3-dioxygenase 3-dioxygenase 1 Bioassay Indoleamine 2 Inhibitor Tryptophan 2

Journal

Bioorganic chemistry
ISSN: 1090-2120
Titre abrégé: Bioorg Chem
Pays: United States
ID NLM: 1303703

Informations de publication

Date de publication:
11 2020
Historique:
received: 19 11 2019
revised: 19 09 2020
accepted: 04 10 2020
pubmed: 5 11 2020
medline: 31 3 2021
entrez: 4 11 2020
Statut: ppublish

Résumé

Inhibitors of indoleamine 2,3-dioxygenase 1 (IDO1) and tryptophan 2,3-dioxygenase (TDO) are potential drugs for the treatment of tumor and neurological diseases. A variety of bioassays have been developed to evaluate IDO1/TDO (IDO1 and/or TDO) inhibitors, with uncertainty regarding how the differences in the assay methods or protocols may influence the assay outcomes. The enzymatic assays of IDO1/TDO are usually performed with NFK assay and Kyn adduct assay while the cellular assays of IDO1 are carried out with Hela assay and HEK293 assay. The present study focused on the comparison of the most common bioassays of IDO1/TDO. In addition, the effects of major factors of bioassays such as reaction time and culture medium on the assay outcomes were evaluated. The study will provide reference for the researchers to select IDO1/TDO inhibitors with bioassays, and promote the development of IDO1/TDO inhibitors.

Identifiants

DOI: 10.1016/j.bioorg.2020.104348 PMID: 33142415
pubmed: 33142415
pii: S0045-2068(20)31646-1
doi: 10.1016/j.bioorg.2020.104348
pii:
doi:

Substances chimiques

Enzyme Inhibitors 0
IDO1 protein, human 0
Indoleamine-Pyrrole 2,3,-Dioxygenase 0
Recombinant Proteins 0
Tryptophan Oxygenase EC 1.13.11.11

Types de publication

Comparative Study Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

104348

Informations de copyright

Copyright © 2020 Elsevier Inc. All rights reserved.

Auteurs

Shengnan Zhang (S)

State Key Laboratory of Genetic Engineering, Department of Biochemistry, School of Life Sciences, Fudan University, Songhu Road 2005, Shanghai 200438, China. Electronic address: 17110700084@fudan.edu.cn.

Leilei Guo (L)

State Key Laboratory of Genetic Engineering, Department of Biochemistry, School of Life Sciences, Fudan University, Songhu Road 2005, Shanghai 200438, China. Electronic address: 17210700113@fudan.edu.cn.

Dan Yang (D)

State Key Laboratory of Genetic Engineering, Department of Biochemistry, School of Life Sciences, Fudan University, Songhu Road 2005, Shanghai 200438, China. Electronic address: 16110700083@fudan.edu.cn.

Zikang Xing (Z)

State Key Laboratory of Genetic Engineering, Department of Biochemistry, School of Life Sciences, Fudan University, Songhu Road 2005, Shanghai 200438, China. Electronic address: 17110700017@fudan.edu.cn.

Weirui Li (W)

State Key Laboratory of Genetic Engineering, Department of Biochemistry, School of Life Sciences, Fudan University, Songhu Road 2005, Shanghai 200438, China. Electronic address: 17210700070@fudan.edu.cn.

Chunxiang Kuang (C)

Shanghai Key Lab of Chemical Assessment and Sustainability, School of Chemical Science and Engineering, Tongji University, 1239 Siping Road, 200092 Shanghai, China. Electronic address: kuangcx@tongji.edu.cn.

Qing Yang (Q)

State Key Laboratory of Genetic Engineering, Department of Biochemistry, School of Life Sciences, Fudan University, Songhu Road 2005, Shanghai 200438, China. Electronic address: yangqing68@fudan.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Dosage biologique › Evaluation and comparison of the commonly used...
questionsmedicales.fr › Phénomènes physiologiques › Phénomènes pharmacologiques et toxicologiques › Phénomènes toxicologiques › Relation dose-effet des médicaments › Evaluation and comparison of the commonly used...
questionsmedicales.fr › Actions chimiques et utilisations › Actions pharmacologiques › Mécanismes moléculaires de l'action pharmacologique › Antienzymes › Evaluation and comparison of the commonly used...
questionsmedicales.fr › Cellules › Cellules épithéliales › Cellules HEK293 › Evaluation and comparison of the commonly used...
questionsmedicales.fr › Cellules › Cellules épithéliales › Cellules HeLa › Evaluation and comparison of the commonly used...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Evaluation and comparison of the commonly used...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Oxidoreductases › Oxygénases › Dioxygenases › Indoleamine-pyrrole 2,3,-dioxygenase › Evaluation and comparison of the commonly used...
questionsmedicales.fr › Phénomènes chimiques › Structure moléculaire › Evaluation and comparison of the commonly used...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines recombinantes › Evaluation and comparison of the commonly used...
questionsmedicales.fr › Phénomènes physiologiques › Phénomènes pharmacologiques et toxicologiques › Phénomènes pharmacologiques › Relation structure-activité › Evaluation and comparison of the commonly used...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Oxidoreductases › Oxygénases › Dioxygenases › Tryptophane 2,3-dioxygenase › Evaluation and comparison of the commonly used...