Ameliorative Effect of Sodium Selenite on Silver Nanoparticles-Induced Myocardiocyte Structural Alterations in Rats.
Animals
Autophagy
Cardiomyopathies
/ chemically induced
Cardiotonic Agents
/ pharmacology
Cell Death
/ drug effects
Dynamins
/ metabolism
Electrocardiography
Male
Metal Nanoparticles
/ adverse effects
Mitochondria, Heart
/ drug effects
Mitochondrial Dynamics
/ drug effects
Mitochondrial Proteins
/ metabolism
Mitophagy
/ drug effects
Myocardium
/ ultrastructure
Myocytes, Cardiac
/ drug effects
Rats, Sprague-Dawley
Silver
/ adverse effects
Sodium Selenite
/ pharmacology
heart
mitochondria
mitochondrial fission
myocardiocyte
myofibril
sarcomere
silver nanoparticles
sodium selenite
Journal
International journal of nanomedicine
ISSN: 1178-2013
Titre abrégé: Int J Nanomedicine
Pays: New Zealand
ID NLM: 101263847
Informations de publication
Date de publication:
2020
2020
Historique:
received:
09
07
2020
accepted:
11
09
2020
entrez:
5
11
2020
pubmed:
6
11
2020
medline:
15
12
2020
Statut:
epublish
Résumé
The application of silver nanoparticles (AgNPs) is growing exponentially, and its potential damage to the cardiac remains to be elucidated. The purpose of this study was to investigate the ameliorative effect of sodium selenite on silver nanoparticles-induced myocardiocyte structural alterations in rats. Forty male Sprague-Dawley (SD) rats were randomly divided into four groups: control group, AgNPs group, Se control group, and AgNPs + Se group. SD rats were administered AgNPs through a single intratracheal instillation, and sodium selenite was given by intraperitoneal injection for seven days. Cardiac function was determined by echocardiography and hemodynamic, ultrastructural changes by transmission electron microscopy examination. Mitochondrial fission and autophagy markers were measured by Western blotting. AgNPs caused a significant decrease in cardiac contraction, diastolic dysfunction, fragmentation, and lysis of the myofibrils, the formation of stenosis in the capillary, damaging the mitochondria membrane and cristae. AgNPs significantly increased mitochondrial fission markers dynamin-related protein 1 (Drp1), phospho-Drp1 (p-Drp1), and mitochondrial fission protein 1 (Fis1), as well as autophagy marker LC3 II/I ( We conclude that the protection of sodium selenite against silver nanoparticles-induced myocardiocyte structural alterations is associated with stabilizing mitochondrial dynamic balance and mitophagy.
Sections du résumé
BACKGROUND
BACKGROUND
The application of silver nanoparticles (AgNPs) is growing exponentially, and its potential damage to the cardiac remains to be elucidated. The purpose of this study was to investigate the ameliorative effect of sodium selenite on silver nanoparticles-induced myocardiocyte structural alterations in rats.
MATERIALS AND METHODS
METHODS
Forty male Sprague-Dawley (SD) rats were randomly divided into four groups: control group, AgNPs group, Se control group, and AgNPs + Se group. SD rats were administered AgNPs through a single intratracheal instillation, and sodium selenite was given by intraperitoneal injection for seven days. Cardiac function was determined by echocardiography and hemodynamic, ultrastructural changes by transmission electron microscopy examination. Mitochondrial fission and autophagy markers were measured by Western blotting.
RESULTS
RESULTS
AgNPs caused a significant decrease in cardiac contraction, diastolic dysfunction, fragmentation, and lysis of the myofibrils, the formation of stenosis in the capillary, damaging the mitochondria membrane and cristae. AgNPs significantly increased mitochondrial fission markers dynamin-related protein 1 (Drp1), phospho-Drp1 (p-Drp1), and mitochondrial fission protein 1 (Fis1), as well as autophagy marker LC3 II/I (
CONCLUSION
CONCLUSIONS
We conclude that the protection of sodium selenite against silver nanoparticles-induced myocardiocyte structural alterations is associated with stabilizing mitochondrial dynamic balance and mitophagy.
Identifiants
pubmed: 33149575
doi: 10.2147/IJN.S271457
pii: 271457
pmc: PMC7603418
doi:
Substances chimiques
Cardiotonic Agents
0
Fis1 protein, rat
0
Mitochondrial Proteins
0
Silver
3M4G523W1G
Dnm1l protein, rat
EC 3.6.5.5
Dynamins
EC 3.6.5.5
Sodium Selenite
HIW548RQ3W
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
8281-8292Informations de copyright
© 2020 Ma et al.
Déclaration de conflit d'intérêts
The authors report no conflicts of interest in this work.
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