Molecular, phylogenetic and antibiotic resistance analysis of enteroaggregative escherichia coli/uropathogenic

Drug Resistance, Microbial Enteropathogenic Escherichia coli / classification Genotype Humans Phylogeny Urinary Tract Infections / microbiology Uropathogenic Escherichia coli / classification
Enteroaggregative Escherichia coli hybrid strain multiplex polymerase chain reaction phylogenetic group serine protease autotransporters of Enterobacteriaceae uropathogenic Escherichia coli

Journal

Indian journal of medical microbiology
ISSN: 1998-3646
Titre abrégé: Indian J Med Microbiol
Pays: United States
ID NLM: 8700903

Informations de publication

Date de publication:
Historique:
entrez: 6 11 2020
pubmed: 7 11 2020
medline: 21 10 2021
Statut: ppublish

Résumé

Horizontal gene transfer of virulence genes (VGs) from different Escherichia coli pathotypes results in the evolution of hybrid strains. Hybrid genotypes of enteroaggregative E. coli and uropathogenic E. coli (EAEC/UPEC) have been reported in sporadic infections and outbreaks of extraintestinal origin. Yet, their association with routine infections is still underrated. In this study, we analysed 163 isolates of E. coli from cases of urinary tract infection seeking hybrid (EAEC/UPEC) strains. Using multiplex polymerase chain reaction, we investigated VGs (adhesive and toxin genes) of UPEC along with EAEC marker genes (aap and agg R), ast A (toxin genes) and serine protease autotransporters of Enterobacteriaceae, pet (plasmid-encoded toxin) and pic (mucinase gene). Those UPEC strains which had characteristic defining genes of EAEC (agg R/aap or their combination) were considered UPEC/EAEC hybrids. Molecular predictors of EAEC (aap and aggR) were detected in 20.2% (33/163) of the strains. The pap C was also detected in 36% of the EAEC/UPEC hybrid strains. Phylogenetic analysis revealed that hybrid strains belonged to Group D (60.6%). Nearly 73.8% of UPEC and 75.7% of UPEC/EAEC hybrid strains were multidrug-resistant. Among UPEC isolates, 72.3% and in hybrid UPEC/EAEC, 78.7% isolates were able to produce biofilm. Our results indicated a closer relationship among EAEC and UPEC, which suggested that some EAEC strains can be potential uropathogens. Ours is a first study documenting the existence of EAEC pathotypes VGs in UPEC strains of nosocomial origin; further studies are required to understand the diarrhoeagenic potential of these hybrids.

Sections du résumé

Background
Horizontal gene transfer of virulence genes (VGs) from different Escherichia coli pathotypes results in the evolution of hybrid strains. Hybrid genotypes of enteroaggregative E. coli and uropathogenic E. coli (EAEC/UPEC) have been reported in sporadic infections and outbreaks of extraintestinal origin. Yet, their association with routine infections is still underrated.
Materials and Methods
In this study, we analysed 163 isolates of E. coli from cases of urinary tract infection seeking hybrid (EAEC/UPEC) strains. Using multiplex polymerase chain reaction, we investigated VGs (adhesive and toxin genes) of UPEC along with EAEC marker genes (aap and agg R), ast A (toxin genes) and serine protease autotransporters of Enterobacteriaceae, pet (plasmid-encoded toxin) and pic (mucinase gene). Those UPEC strains which had characteristic defining genes of EAEC (agg R/aap or their combination) were considered UPEC/EAEC hybrids.
Results
Molecular predictors of EAEC (aap and aggR) were detected in 20.2% (33/163) of the strains. The pap C was also detected in 36% of the EAEC/UPEC hybrid strains. Phylogenetic analysis revealed that hybrid strains belonged to Group D (60.6%). Nearly 73.8% of UPEC and 75.7% of UPEC/EAEC hybrid strains were multidrug-resistant. Among UPEC isolates, 72.3% and in hybrid UPEC/EAEC, 78.7% isolates were able to produce biofilm.
Conclusions
Our results indicated a closer relationship among EAEC and UPEC, which suggested that some EAEC strains can be potential uropathogens. Ours is a first study documenting the existence of EAEC pathotypes VGs in UPEC strains of nosocomial origin; further studies are required to understand the diarrhoeagenic potential of these hybrids.

Identifiants

DOI: 10.4103/ijmm.IJMM_20_365 PMID: 33154257
pubmed: 33154257
pii: IndianJMedMicrobiol_2020_38_3_421_299841
doi: 10.4103/ijmm.IJMM_20_365
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

421-429

Déclaration de conflit d'intérêts

None

Auteurs

Vinay Modgil (V)

Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.

Harpreet Kaur (H)

Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.

Balvinder Mohan (B)

Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.

Neelam Taneja (N)

Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.

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Classifications MeSH

questionsmedicales.fr › Phénomènes physiologiques › Phénomènes pharmacologiques et toxicologiques › Phénomènes pharmacologiques › Résistance aux substances › Résistance microbienne aux médicaments › Molecular, phylogenetic and antibiotic...
questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Enterobacteriaceae › Escherichia › Escherichia coli › Escherichia coli entéropathogène › Molecular, phylogenetic and antibiotic...
questionsmedicales.fr › Phénomènes génétiques › Génotype › Molecular, phylogenetic and antibiotic...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Molecular, phylogenetic and antibiotic...
questionsmedicales.fr › Sciences de l'information › Phylogenèse › Molecular, phylogenetic and antibiotic...
questionsmedicales.fr › Maladies urogénitales › Maladies urologiques › Infections urinaires › Molecular, phylogenetic and antibiotic...
questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Enterobacteriaceae › Escherichia › Escherichia coli › Escherichia coli pathogènes extra-intestinales › Escherichia coli uropathogène › Molecular, phylogenetic and antibiotic...