Absence of Toxemia in Clostridioides difficile Infection: Results from Ultrasensitive Toxin Assay of Serum.
Antibiotic-associated diarrhea
Clostridioides difficile
Colitis
Infectious diarrhea
Nosocomial infection
Simoa ultrasensitive toxin assay
Journal
Digestive diseases and sciences
ISSN: 1573-2568
Titre abrégé: Dig Dis Sci
Pays: United States
ID NLM: 7902782
Informations de publication
Date de publication:
10 2021
10 2021
Historique:
received:
13
08
2020
accepted:
15
10
2020
pubmed:
10
11
2020
medline:
11
11
2021
entrez:
9
11
2020
Statut:
ppublish
Résumé
Clostridioides difficile infection (CDI) is caused by Toxins A and B, secreted from pathogenic strains of C. difficle. This infection can vary greatly in symptom severity and in clinical presentation. Current assays used to diagnose CDI may lack the required sensitivity to detect the exotoxins circulating in blood. The ultrasensitive single molecule array (Simoa) assay was modified to separately detect toxin A and toxin B in serum with a limit of detection at the low picogram level. When applied to a diverse cohort, Simoa was unable to detect toxins A or B in serum from patients with CDI, including many classified as having severe disease. The detection of toxin may be limited by the inference of antitoxin antibodies circulating in serum. This result does not support the hypothesis that toxemia occurs in C. difficile infection, conflicting with the findings of other published reports.
Identifiants
pubmed: 33164145
doi: 10.1007/s10620-020-06683-8
pii: 10.1007/s10620-020-06683-8
pmc: PMC8105423
mid: NIHMS1645012
doi:
Substances chimiques
Bacterial Proteins
0
Bacterial Toxins
0
Enterotoxins
0
Immunoglobulin A
0
Immunoglobulin G
0
tcdA protein, Clostridium difficile
0
toxB protein, Clostridium difficile
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
3303-3306Subventions
Organisme : NIAID NIH HHS
ID : R01 AI116596
Pays : United States
Informations de copyright
© 2020. Springer Science+Business Media, LLC, part of Springer Nature.
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