A novel variant in COX16 causes cytochrome c oxidase deficiency, severe fatal neonatal lactic acidosis, encephalopathy, cardiomyopathy, and liver dysfunction.


Journal

Human mutation
ISSN: 1098-1004
Titre abrégé: Hum Mutat
Pays: United States
ID NLM: 9215429

Informations de publication

Date de publication:
02 2021
Historique:
received: 16 07 2020
revised: 26 10 2020
accepted: 02 11 2020
pubmed: 11 11 2020
medline: 1 4 2022
entrez: 10 11 2020
Statut: ppublish

Résumé

COX16 is involved in the biogenesis of cytochrome-c-oxidase (complex IV), the terminal complex of the mitochondrial respiratory chain. We present the first report of two unrelated patients with the homozygous nonsense variant c.244C>T(p. Arg82*) in COX16 with hypertrophic cardiomyopathy, encephalopathy and severe fatal lactic acidosis, and isolated complex IV deficiency. The absence of COX16 protein expression leads to a complete loss of the holo-complex IV, as detected by Western blot in patient fibroblasts. Lentiviral transduction of patient fibroblasts with wild-type COX16 complementary DNA rescued complex IV biosynthesis. We hypothesize that COX16 could play a role in the copper delivery route of the COX2 module as part of the complex IV assembly. Our data provide clear evidence for the pathogenicity of the COX16 variant as a cause for the observed clinical features and the isolated complex IV deficiency in these two patients and that COX16 deficiency is a cause for mitochondrial disease.

Identifiants

pubmed: 33169484
doi: 10.1002/humu.24137
pmc: PMC7898715
doi:

Substances chimiques

COX16 protein, human 0
Membrane Proteins 0
Mitochondrial Proteins 0

Types de publication

Case Reports Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

135-141

Informations de copyright

© 2020 The Authors. Human Mutation published by Wiley Periodicals LLC.

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Auteurs

Liesbeth T M Wintjes (LTM)

Department of Laboratory Medicine, Translational Metabolic Laboratory, Radboud Centre for Mitochondrial Medicine, Radboudumc, Nijmegen, The Netherlands.

Maina Kava (M)

Department of Neurology, Perth Children's Hospital, Perth, Western Australia, Australia.
School of Pediatrics and Child Health, University of Western Australia, Perth, Western Australia, Australia.

Frans A van den Brandt (FA)

Department of Laboratory Medicine, Translational Metabolic Laboratory, Radboud Centre for Mitochondrial Medicine, Radboudumc, Nijmegen, The Netherlands.

Mariël A M van den Brand (MAM)

Department of Pediatrics, Radboud Centre for Mitochondrial Medicine, Radboudumc, Nijmegen, The Netherlands.

Oksana Lapina (O)

Department for Radiology and Nuclear Medicine, Oslo University Hospital, Oslo, Norway.

Yngve T Bliksrud (YT)

Norwegian National Unit for Diagnostics of Congenital Metabolic Disorders, Department of Medical Biochemistry, Oslo University Hospital, Oslo, Norway.

Mari A Kulseth (MA)

Department of Medical Genetics, Oslo University Hospital, Oslo, Norway.

Silja S Amundsen (SS)

Department of Medical Genetics, Oslo University Hospital, Oslo, Norway.

Terje R Selberg (TR)

Department of Pediatrics, Ostfold Hospital Trust, Kalnes, Norway.

Marion Ybema-Antoine (M)

Department of Pediatrics, Radboud Centre for Mitochondrial Medicine, Radboudumc, Nijmegen, The Netherlands.

Omar A Z Tutakhel (OAZ)

Department of Laboratory Medicine, Translational Metabolic Laboratory, Radboud Centre for Mitochondrial Medicine, Radboudumc, Nijmegen, The Netherlands.

Lawrence Greed (L)

Department of Clinical Biochemistry, PathWest, Perth, Western Australia, Australia.

David R Thorburn (DR)

Murdoch Children's Research Institute and Victorian Clinical Genetics Services, Royal Children's Hospital, Melbourne, Victoria, Australia.
Department of Pediatrics, University of Melbourne, Melbourne, Victoria, Australia.

Trine Tangeraas (T)

Norwegian National Unit for Newborn Screening, Division of Pediatric and Adolescent Medicine, Oslo University Hospital, Oslo, Norway.

Shanti Balasubramaniam (S)

Western Sydney Genetics Program, The Children's Hospital at Westmead, Sydney, New South Wales, Australia.

Richard J T Rodenburg (RJT)

Department of Laboratory Medicine, Translational Metabolic Laboratory, Radboud Centre for Mitochondrial Medicine, Radboudumc, Nijmegen, The Netherlands.
Department of Pediatrics, Radboud Centre for Mitochondrial Medicine, Radboudumc, Nijmegen, The Netherlands.

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